Invitrogen

TNIP1 Monoclonal Antibody (1A11E3)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

TNIP1 Antibody in Western Blot (WB) — Western blot analysis of ABIN-1 was performed using whole cell extracts (30 µg lysate) of A-431 (Lane 1), PC-3 (Lane 2), HEL 92.1.7 (Lane 3) and K-562 (Lane 4). The blots were probed with Anti-ABIN-1 Mouse monoclonal Antibody (Product # 37-6100, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 72 kDa band corresponding to ABIN-1 was observed across the cell lines tested. A lower band of 48 kDa was observed showing NEMO/IKK gamma which binds in complex. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

37-6100

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 4 publications 4 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	1A11E3
Immunogen	Synthetic peptide derived from the N-terminal region of the human, mouse and rat ABIN-1 protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533330

Target Information

Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TNF receptor-activated factor 2 mediates cardiac protection through noncanonical NF-κB signaling.

JCI insight

Evans S,Tzeng HP,Veis DJ,Matkovich S,Weinheimer C,Kovacs A,Barger PM,Mann DL

37-6100 was used in Western Blotting to elucidate the mechanisms responsible for the cytoprotective effects of TNF receptor-activated factor 2 in the heart.

Thu Feb 08 00:00:00 EST 2018

The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes.

EMBO reports

Afonina IS,Van Nuffel E,Baudelet G,Driege Y,Kreike M,Staal J,Beyaert R

376100 was used in western blot to show that CARD14 activates p38 and JNK MAP kinase pathways

Wed Jun 01 00:00:00 EDT 2016

Hematopoietic progenitor cell lines with myeloid and lymphoid potential.

Nature methods

Redecke V,Wu R,Zhou J,Finkelstein D,Chaturvedi V,High AA,Häcker H

37-6100 was used in flow cytometry to generate and characterize Hoxb8-FL cells

Thu Aug 01 00:00:00 EDT 2013

ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.

The Journal of biological chemistry

Gao L,Coope H,Grant S,Ma A,Ley SC,Harhaj EW

Fri Oct 21 00:00:00 EDT 2011

A large scale shRNA barcode screen identifies the circadian clock component ARNTL as putative regulator of the p53 tumor suppressor pathway.

PloS one

Mullenders J,Fabius AW,Madiredjo M,Bernards R,Beijersbergen RL

37-6100 was used in western blot to identify novel genes that regulate p53 function

Thu Jul 02 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; Naf1; Nef-associated factor 1; Nef-associated factor 1 SNP; Nip40-1; TNFAIP3-interacting protein 1; VAN; Virion-associated nuclear shuttling protein

Gene Aliases: ABIN-1; KIAA0113; NAF1; nip40-1; TNIP1; VAN

UniProt ID: (Human) Q15025

Entrez Gene ID: (Human) 10318

Function(s)

MyD88-dependent toll-like receptor signaling pathway translation proteolysis defense response inflammatory response leukocyte cell-cell adhesion glycoprotein biosynthetic process negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of viral genome replication positive regulation of transcription from RNA polymerase II promoter positive regulation of inflammatory response negative regulation of ERK1 and ERK2 cascade modulation by symbiont of host I-kappaB kinase/NF-kappaB cascade

Process(es)

thiol-dependent ubiquitin-specific protease activity protein binding mitogen-activated protein kinase binding

TNIP1 Monoclonal Antibody (1A11E3)

Product Details

37-6100

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!