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Immunofluorescence analysis of CRMP2 was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CRMP2 Rabbit Polyclonal Antibody (PA3120) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membranous localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Y(499) D G P V F D L T T T P K(511) of TUC-4a (residues 612-624 of TUC-4b).|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-120 detects TOAD-64 in rat samples.
PA3-120 has been successfully used in Western blot, immunoprecipitation and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects an 64 kDa protein representing TOAD-64 in rat samples.
The PA3-120 immunogen is a synthetic peptide corresponding to residues Y(499) D G P V F D L T T T P K(511) of TUC-4a (residues 612-624 of TUC-4b).
TOAD-64 is a 64 kDa intracellular neural protein, which belongs to a family of genes that inhibit nerve growth. It also appears to be involved in a role of axon outgrowth. Preliminary results indicate inhibition of TOAD-64 expression poteintiates neurite outgrowth, and may lead to the design of therapies that promote axonal regeneration. Antibodies to TOAD-64 render neurons insensitive to extracellular compounds that inhibit neuron growth. Very few reagents are able to identify newly born neurons as well as antibodies to TOAD-64.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
collapsin response mediator protein 2; CRMP-2; dihydropyrimidinase-related protein 2; DPYL2; DRP-2; DRP2; Turned On After Division 64; turned on after division 64 kDa protein; turned on after division, 64 kDa protein; ULIP-2; unc-33-like phosphoprotein 2
AI851130; Crmp2; Dpysl2; DRP2; Musunc33; TOAD-64; Ulip2