Immunofluorescent analysis of TOMM22 showing co-staining of mitochondria and ER-Golgi intermediate compartment in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100 and stained with a TOMM2 monoclonal antibody (Product # MA1-20161) at 2 µg/mL. Detection was performed with an Atto 488 Conjugate (green) and 1 µg/mL Rabbit anti-ERGIC-53/p58, Cy3 Conjugate (ER-Golgi intermediate compartment stained in red). Nuclei were counterstained with DAPI (blue).
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Membrane fraction from Vero (monkey kidney-derived) cells.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-20161 detects TOMM22 in human and non-human primate samples.
MA1-20161 has been successfully used in immunocytochemistry and Western blot procedures.
The MA1-20161 immunogen is a membrane fraction from Vero (monkey kidney-derived) cells.
Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reciprocal effects of alpha-synuclein overexpression and proteasome inhibition in neuronal cells and tissue.
MA1-20161 was used in immunocytochemistry to study the relation between alpha-synuclein overexpression and proteasome inhibition in neuronal cells and tissue
|Dyllick-Brenzinger M,D'Souza CA,Dahlmann B,Kloetzel PM,Tandon A||Neurotoxicity research (17:215)||2010|