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TOP2A Antibody (MA5-44952) in IHC (P)
Immunohistochemistry analysis of Topoisomerase II alpha in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with Topoisomerase II alpha Monoclonal antibody (Product # MA5-44952) using... View More
Product Details
MA5-44952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A3F12
Immunogen
Synthetic peptide within human Topoisomerase II alpha aa1480-1531.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931408
Target Information
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
Gene Aliases: Top-2; TOP2; TOP2A; TP2A
UniProt ID: (Human) P11388, (Mouse) Q01320
Entrez Gene ID: (Human) 7153, (Mouse) 21973
magnesium ion binding
DNA binding
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
protein C-terminus binding
DNA-dependent ATPase activity
drug binding
DNA binding, bending
enzyme binding
protein homodimerization activity
histone deacetylase binding
ubiquitin binding
poly(A) RNA binding
protein heterodimerization activity
nucleotide binding
DNA topoisomerase activity
isomerase activity
sequence-specific DNA binding
structure-specific DNA binding
metal ion binding
DNA metabolism protein
resolution of meiotic recombination intermediates
sister chromatid segregation
hematopoietic progenitor cell differentiation
DNA topological change
DNA ligation
DNA unwinding involved in DNA replication
mitotic recombination
cellular response to DNA damage stimulus
chromosome segregation
protein sumoylation
apoptotic chromosome condensation
embryonic cleavage
regulation of circadian rhythm
positive regulation of apoptotic process
mitotic DNA integrity checkpoint
positive regulation of viral genome replication
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
DNA metabolic process
chromosome condensation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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