Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
COT (Cancer Osaka thyroid oncogene) also known as MAP3K8/TPL2 (Mitogen Activated Protein kinase 8/Tumor Progression Locus 2). This oncogene is a member of the serine threonine protein kinase family and is localized in the cytoplasm. It is known to activate JNK kinase and MAP kinase pathways along with activating IK-B kinases aiding in nuclear production of NF-KappaB. The production of IL-2 and TNFalpha during T cell activation is also mediated by COT/MAP3K8/TPL2. In addition to this, COT/MAP3K8/TPL2 also aids in T-cell costimulation, signal transduction, and amino acid phosphorylation. In humans, the MAP3K8 gene encoding COT/MAP3K8/TPL2 is present on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: augmented in rheumatoid arthritis 2; c-COT; Cancer Osaka thyroid oncogene; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; MEKK8; Mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL-2; TPL2; Tumor progression locus 2
Gene Aliases: AURA2; c-COT; COT; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
UniProt ID: (Human) P41279
Entrez Gene ID: (Human) 1326