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|Tested species reactivity||Chicken, Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Tropomyosin protein purified from chicken embryo fibroblasts (CEF) and chicken leg, heart, and gizzard|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-11783 targets Tropomyosin in IF and IHC (P) applications and shows reactivity with Chicken, Human, and Rat samples.
The MA5-11783 immunogen is tropomyosin protein purified from chicken embryo fibroblasts (CEF) and chicken leg, heart, and gizzard.
Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Degradation of p21Cip1 through anaphase-promoting complex/cyclosome and its activator Cdc20 (APC/CCdc20) ubiquitin ligase complex-mediated ubiquitylation is inhibited by cyclin-dependent kinase 2 in cardiomyocytes.
MA5-11783 was used in immunohistochemistry to study the role of ubiquitin-mediated degradation of p21Cip1 in cell cycle arrest and terminal differentiation
|Yamada K,Tamamori-Adachi M,Goto I,Iizuka M,Yasukawa T,Aso T,Okazaki T,Kitajima S||The Journal of biological chemistry (286:44057)||2011|
alpha tropomyosin; Alpha-tropomyosin; alpha-tropomyosin 1; alpha-tropomyosin 2; alpha-tropomyosin of skeletal fast muscle; cardiomyopathy; cardiomyopathy, hypertrophic 3; epididymis secretory protein Li 265; hepatoma alpha tropomyosin; hypertrophic 3; sarcomeric tropomyosin kappa; smooth muscle alpha-tropomyosin; striated muscle alpha-tropomyosin; tropomyosin (CTm4); tropomyosin (CTm7); tropomyosin 3 alpha; Tropomyosin alpha-1 chain; Tropomyosin-1
alpha-fTM; Alpha-tm; C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; Tma2; TMSA; TPM1; Tpma