Immunohistochemistry was performed on biopsies of deparaffinized Human testis tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing TPX2 (MA1-802) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant human TPX2|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||4 ug/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-802 detects the TPX2 in human, rat and mouse cells.
MA1-802 has been successfully used in Western blot, immunoprecipitation and immunofluorescence procedures. By Western blot, this antibody detects a 92 kDa protein.
The MA1-802 immunogen is recombinant human TPX2.
TPX2 is essential for spindle pole and mitotic spindle formulation, playing a role in the S and G2 phases of mitosis. It is also critical for the function of protein kinase Aurora A. TPX2 autophosphorylates Aurora A, targeting it to the spindle apparatus and allowing it to function in eukaryotic meiotic and mitotic cell cycles. TPX2 has also been found to be highly expressed in cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.