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Imunofluorescent analysis of TRA-1-60 (red) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 650-conjugated TRA-1-60 monoclonal antibody (bottom right and top left panels, Product # MA1-023-D650) or DyLight 650-conjugated Mouse IgM isotype control antibody (top right panel, Product # MA1-194-D650), at a dilution of 1:250 and incubated for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on an EVOS FL Imaging system at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Human embryonal carcinoma cell line 2102Ep.|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-023-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.
TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.