Western blot analysis of TRA-1-81 was performed by loading 50ug of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||human embryonal carcinoma cell line 2102Ep|
|Purification||Affinity chromatography - MBP|
|Storage buffer||proprietary buffer with proprietary stabilizer|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-024-HRP has been successfully used in Western blot applications on human samples.
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.