Immunofluorescence analysis of TRAF3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ABfinity TRAF3 Recombinant Rabbit Monoclonal Antibody (700121) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization and panel e is a no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 1-187 of Q13114.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||0.5-1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, porcine, canine, bovine and equine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
TRAF3, also known as TNF receptor-associated factor 3, is an adapter protein and signal transducer that links multiple members of the tumor necrosis receptor family to different signaling pathways. This is done by association with the receptor cytoplasmic domain and various kinases. It also regulates innate immune system by modulating signaling via Toll-like receptors. Overexpression of TRAF3 does not induce activation of the commonly known TRAF-inducible signaling pathways, NF- kappaB and JNK. In contrast, it acts as a negative regulator of the NF- kappaB pathway. Furthermore, TRAF3 is a positive regulator of type I IFN production. These results indicate that TRAF3 is a critical regulator of both innate and adaptive immune responses. In addition to being a major modulator of immune responses, TRAF3 regulates B cell homeostasis in secondary lymphoid organs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Cellular inhibitors of apoptosis are global regulators of NF-¿B and MAPK activation by members of the TNF family of receptors.||Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D||Science signaling (5:null)||2012|
|Human||Not Cited||Cellular inhibitors of apoptosis are global regulators of NF-¿B and MAPK activation by members of the TNF family of receptors.||Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D||Science signaling (5:null)||2012|