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|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: Y(24) S A T T A R Q E E V P(35) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
PA1-956 detects decoy receptor 1 (DcR1) from human and rat tissues and cells.
PA1-956 has been successfully used in FACS and Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing DcR1 from HEK293 cell lysate.
PA1-956 immunizing peptide corresponds to amino acid residues 24-35 of human DcR1. PA1-956 immunizing peptide (Cat. # PEP-047) is available for use in neutralization and control experiments.
DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human retinoblastoma cells are resistant to apoptosis induced by death receptors: role of caspase-8 gene silencing.
PA1-956 was used in flow cytometry to investigate the role of caspase-8 gene silencing on apoptosis in human Rb cell lines.
|Poulaki V,Mitsiades CS,McMullan C,Fanourakis G,Negri J,Goudopoulou A,Halikias IX,Voutsinas G,Tseleni-Balafouta S,Miller JW,Mitsiades N||Investigative ophthalmology & visual science (46:358)||2005|
Antagonist decoy receptor for TRAIL/Apo-2L; CD263; cytotoxic TRAIL receptor-3; DCR1; Decoy receptor 1; Decoy TRAIL receptor without death domain; LIT; Lymphocyte inhibitor of TRAIL; MGC149501; MGC149502; TNF-related apoptosis-inducing ligand receptor 3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRID; tumor necrosis factor ligand superfamily member 10; Tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
CD263; DCR1; DCR1-TNFR; LIT; TNFRSF10C; Trail; TRAIL-R3; TRAILR3; TRID; UNQ321/PRO366