Invitrogen

TREM1 Monoclonal Antibody (L5-B8.2A12.3A12)

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TREM1 Antibody in Flow Cytometry (Flow) — Fig. 3 TREM-1-mediated IL-1beta induction by M1 macrophages elicits IL-22 production by ILC3. a Contour plot of FACS analysis data showed the purity of AutoMACS-purified ILCs b ELISA determination of IL-22 production by AutoMACS-purified WT Lin - CD45 + ILCs that were co-cultured with WT GM-BMDMs in the presence or absence of the indicated murine recombinant cytokines. Culture supernatants were harvested after 48 h. Mock, no treatment. ILCs treated with IL-1beta + IL-23 in the absence of macrophages served as positive control and IFNgamma + LPS or IL-4 + IL-13 in the absence of macrophages served as negative control. Data are the mean +- SEM and representative of four independent experiments performed in triplicate. c ELISA determination of IL-22 production by AutoMACS-purified WT Lin - CD45 + ILCs that were co-cultured with WT or TREM-1 KO cells (GM-BMDMs or neutrophils, respectively) in the presence of LPS or LPS plus anti-TREM-1 agonistic antibodies (TR1 Ab) or isotype antibodies, respectively. Data are mean +- SEM and representative of 3 independent experiments performed in triplicate. Histogram of FACS analysis data showed TREM-1 levels on M- or GM- BMDMs. d-e Real-time PCR analysis of mRNA level of IL-1beta and ELISA determination of IL-1beta production by WT- or TREM-1 KO- GM-BMDMs that were cultured in the presence of the polarizing cytokines (IFNgamma + LPS for M1 or IL-4 + IL-13 for M2). Culture supernatants were harvested after 24 h. Mock, no treatment. For ( b-e )

Product Details

MA5-16765

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	1:25-1:100	View 1 publication 1 publication
Functional Assay (FN)	Assay-dependent	-

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	L5-B8.2A12.3A12
Immunogen	Mouse TREM-1 Fc fusion protein
Conjugate	Unconjugated PE
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2538248

Product Specific Information

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.

Rat anti Mouse Trem-1 antibody, clone L5-B8.2A12.3A12 recognizes mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes.

Target Information

TREM1 (triggering receptor expressed on myeloid cells 1) is a receptor belonging to the Ig superfamily that amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. It is thought that TREM1 other related TREM proteins such as TREM2 act to modulate the inflammatory response. TREM1 has also been linked to inflammatory bowel disease resulting in enhanced production of TNF, IL-6, IL-8, and MCP-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.

Journal of biomedical science

Yang FC,Chiu PY,Chen Y,Mak TW,Chen NJ

Published figure using TREM1 monoclonal antibody (Product # MA5-16765) in Flow Cytometry

Wed Jun 12 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD354; sCD354; soluble CD354; soluble TREM 1; soluble TREM1; sTREM 1; sTREM1; TREM; TREM-1; triggering receptor expressed in monocytes 1; Triggering receptor expressed on myeloid cells; Triggering receptor expressed on myeloid cells 1

Gene Aliases: Trem1

UniProt ID: (Mouse) Q9JKE2

Entrez Gene ID: (Mouse) 58217

Function(s)

positive regulation of defense response to virus by host cytokine secretion involved in immune response cell migration neutrophil chemotaxis cytokine metabolic process chemokine metabolic process neutrophil mediated killing of gram-negative bacterium neutrophil extravasation mitophagy in response to mitochondrial depolarization

Process(es)

scaffold protein binding

TREM1 Monoclonal Antibody (L5-B8.2A12.3A12)

Product Details

MA5-16765

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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