|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Amino acids 1099-1372 of Trip230 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23294 detects TRIP230 in Human samples.
MA1-23294 has been successfully used in Immunoprecipitation, immunocytochemistry, immunofluorescence and Western Blot procedures.
MA1-23294 immunogen corresponds to Amino acids 1099-1372 of Trip230 expressed in E. coli..
The Trip230 gene encodes a protein of 1,978 amino acids with an estimated molecular mass of 230 kDa. The gene is located on chromosome 14q31, which is in a region associated with genetic alterations and thyroid hormone response abnormalities. The Trip230 protein interacts with Rb and thyroid hormone receptor (TR) using two distinct domains. Complex formation with Rb is in a hormone independent manner, but when Trip230 forms a complex with TR, it is in a thyroid hormone-dependent manner. After T3 treatment, Trip230 is phosphorylated and transported to the nucleus, suggesting that phosphorylation may be important for its nuclear translocation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.