Invitrogen

TRPM7 Monoclonal Antibody (S74)

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TRPM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TRPM7 in paraffin-embedded mouse hippocampus. Samples were incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.

TRPM7 Antibody in Western Blot (WB) — Western blot analysis of TRPM7 in human cell lysates with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).

TRPM7 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 EGF enhances direct interaction between EGFR and TRPM7 in a c-Src-dependent manner ( A ) EGFR was detected when TRPM7 was immunoprecipitated in rVSMCs using anti-TRPM7 antibody (left panel) and TRPM7 was detected when EGFR was immunoprecipitated using anti-EGFR antibody (right panel) ( n =3-4). ( B ) Representative confocal microscopy images of rVSMCs co-immunostained for TRPM7 (Alexa 488, green) and EGFR (Alexa 555, red). Nuclei were stained with DAPI ( n =3). ( C , D ) PLA was used to visualize and quantify TRPM7-EGFR interaction in rVSMCs stimulated with vehicle (veh), and EGF (50 ng/ml) in the presence and absence of gefitinib (1 uM) and PP2 (10 uM). WGA (Alexa 488, green) was used to stain cell membranes. Red fluorescence is identified as PLA positive signals. Orange fluorescence in the merged figure identifies co-localization. PLA signals were quantified using the Analyse Particles plugin of ImageJ software. For each condition, quantifications were performed from at least 100 cells and expressed as the mean number of signals per cell (percentage of control). Scale bar =10 um. Arrows indicate co-localization of EGFR and TRPM7. * P <0.05 compared with vehicle control (veh, PBS) and + P <0.05 compared with EGF-treated cells.

Product Details

MA5-27620

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	S74
Immunogen	Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7 View immunogen
Conjugate	Unconjugated APC FITC PE PerCP
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20°C
RRID	AB_2735401

Target Information

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics.

eLife

Kollewe A,Chubanov V,Tseung FT,Correia L,Schmidt E,Rössig A,Zierler S,Haupt A,Müller CS,Bildl W,Schulte U,Nicke A,Fakler B,Gudermann T

MA5-27620 was used in Immunoprecipitation to investigate the subunit composition of endogenous TRPM7 channels in rodent brain by multi-epitope affinity purification and high-resolution quantitative mass spectrometry analysis.

Fri Nov 12 00:00:00 EST 2021

Epidermal growth factor signaling through transient receptor potential melastatin 7 cation channel regulates vascular smooth muscle cell function.

Clinical science (London, England : 1979)

Zou ZG,Rios FJ,Neves KB,Alves-Lopes R,Ling J,Baillie GS,Gao X,Fuller W,Camargo LL,Gudermann T,Chubanov V,Montezano AC,Touyz RM

MA5-27620 was used in Immunocytochemistry to determine whether TRPM7 is a downstream target of EGF in VSMCs and if EGF receptor through TRPM7 influences VSMC function.

Fri Aug 14 00:00:00 EDT 2020

Epidermal growth factor signaling through transient receptor potential melastatin 7 cation channel regulates vascular smooth muscle cell function.

Clinical science (London, England : 1979)

Zou ZG,Rios FJ,Neves KB,Alves-Lopes R,Ling J,Baillie GS,Gao X,Fuller W,Camargo LL,Gudermann T,Chubanov V,Montezano AC,Touyz RM

MA5-27620 was used in Immunocytochemistry to determine whether TRPM7 is a downstream target of EGF in VSMCs and if EGF receptor through TRPM7 influences VSMC function.

Fri Aug 14 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Channel kinase 1; Channel-kinase 1; FLJ20117; Long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250|UniProtKB:Q96QT4}; LTRPC ion channel family member 7; LTrpC-7; LTrpC7 {ECO:0000250|UniProtKB:Q96QT4}; tr; Transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312|RGD:620053}; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; Transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303|PubMed:11161216}; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; TRP-PLIK; TRP-PLIK {ECO:0000303|PubMed:11161216}; trpm7 {ECO:0000312|RGD:620053}

Gene Aliases: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPM7; TRPPLIK

UniProt ID: (Human) Q96QT4, (Mouse) Q923J1

Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906

Molecular Function: ion channel transporter