Invitrogen

TSG101 Monoclonal Antibody (4A10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

14 Published Figures

21 References

View all (26) TSG101 antibodies

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Datasheet

Protocols

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TSG101 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — TSG101 Monoclonal Antibody (4A10) detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 Monoclonal Antibody (4A10) (Product # MA1-23296) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TSG101 Antibody in Western Blot (WB) — Western Blot using TSG101 Monoclonal Antibody (4A10) (Product # MA1-23296). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 Monoclonal Antibody (4A10) (Product # MA1-23296) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TSG101 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of RACGAP1 was performed in THP-1 cells using RACGAP1 Polyclonal Antibody (Product # PA1-31510) (red) at a dilution of 1:25. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

TSG101 Antibody in ELISA (ELISA) — ELISA detection of TSG101 using TSG101 Polyclonal Antibody (Product # PA5-31260) for capture at a concentration of 5 µg/mL and TSG101 Monoclonal Antibody (4A10) (Product # MA1-23296) for detection at a concentration of 1.5 µg/mL.

TSG101 Antibody in Immunocytochemistry (ICC/IF) — Fig. 5 Cellular uptake assay in HUVECs. Confocal imaging of EVs labeled with antibodies to CD63, TSG101, calnexin along with PKH67 dye. The uptake of CD63 (upper panel) and TSG101 antibodies (lower panel) labeled EVs along with PKH67 dye molecules were analysed in HUVECs after 6 h of incubation with EVs ( a and b ). Calnexin and PKH67 dual staining served as a negative control to ensure specificity of signal ( c ). EVs labeled with only secondary antibody and PKH67 dye served as negative control ( d )

TSG101 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot and Immunoprecipitation

TSG101 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with TSG101 siRNA and decrease in signal intensity was observed in western blot application using TSG101 (Product # MA1-23296). {KD}

Product Details

MA1-23296

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 19 publications 19 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:500-1:1,000	View 1 publication 1 publication
Flow Cytometry (Flow)	1:25-1:200	-
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Immunomicroscopy (IM)	1:10	-

Product Specifications
Species Reactivity	Hamster, Human, Mouse, Non-human primate, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	4A10
Immunogen	Residues 167-374 of TSG101, expressed in E. coli. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.8 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2208088

Product Specific Information

Suggested positive controls for this product are MOLT4 and HeLa.

Target Information

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Sat Feb 19 00:00:00 EST 2022

Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization.

BMC gastroenterology

Xia W,Lu Z,Chen W,Zhou J,Zhao Y

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Sat Feb 19 00:00:00 EST 2022

TKI-Resistant Renal Cancer Secretes Low-Level Exosomal miR-549a to Induce Vascular Permeability and Angiogenesis to Promote Tumor Metastasis.

Frontiers in cell and developmental biology

Xuan Z,Chen C,Tang W,Ye S,Zheng J,Zhao Y,Shi Z,Zhang L,Sun H,Shao C

MA1-23296 was used in Western Blot to study the effect of cancer-derived exosomes on metastasis mechanisms in tyrosine-kinase inhibitor-resistant renal cancer.

Thu Aug 05 00:00:00 EDT 2021

TKI-Resistant Renal Cancer Secretes Low-Level Exosomal miR-549a to Induce Vascular Permeability and Angiogenesis to Promote Tumor Metastasis.

Frontiers in cell and developmental biology

Xuan Z,Chen C,Tang W,Ye S,Zheng J,Zhao Y,Shi Z,Zhang L,Sun H,Shao C

MA1-23296 was used in Western Blot to study the effect of cancer-derived exosomes on metastasis mechanisms in tyrosine-kinase inhibitor-resistant renal cancer.

Thu Aug 05 00:00:00 EDT 2021

Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells.

Frontiers in cellular and infection microbiology

Elsayed R,Elashiry M,Liu Y,El-Awady A,Hamrick M,Cutler CW

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Mon Jul 05 00:00:00 EDT 2021

Generation and Application of a Reporter Cell Line for the Quantitative Screen of Extracellular Vesicle Release.

Frontiers in pharmacology

Shpigelman J,Lao FS,Yao S,Li C,Saito T,Sato-Kaneko F,Nolan JP,Shukla NM,Pu M,Messer K,Cottam HB,Carson DA,Corr M,Hayashi T

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Tue May 04 00:00:00 EDT 2021

Cancer-secreted miRNAs regulate amino-acid-induced mTORC1 signaling and fibroblast protein synthesis.

EMBO reports

Fong MY,Yan W,Ghassemian M,Wu X,Zhou X,Cao M,Jiang L,Wang J,Liu X,Zhang J,Wang SE

MA1-23296 was used in Western Blot to suggest that through educating fibroblasts to reduce and re-prioritize mRNA translation, cancer cells rewire the metabolic fluxes of amino acid pool and dynamically regulate stroma-produced proteins during periodic nutrient fluctuations.

Wed Feb 03 00:00:00 EST 2021

Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles.

Cancers

Palviainen M,Laukkanen K,Tavukcuoglu Z,Velagapudi V,Kärkkäinen O,Hanhineva K,Auriola S,Ranki A,Siljander P

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Fri Nov 06 00:00:00 EST 2020

Exosomes From Astrocyte Processes: Signaling to Neurons.

Frontiers in pharmacology

Venturini A,Passalacqua M,Pelassa S,Pastorino F,Tedesco M,Cortese K,Gagliani MC,Leo G,Maura G,Guidolin D,Agnati LF,Marcoli M,Cervetto C

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells.

Biological procedures online

Mondal A,Ashiq KA,Phulpagar P,Singh DK,Shiras A

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells.

Biological procedures online

Mondal A,Ashiq KA,Phulpagar P,Singh DK,Shiras A

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Evidence for Enhanced Exosome Production in Aromatase Inhibitor-Resistant Breast Cancer Cells.

International journal of molecular sciences

Augimeri G,La Camera G,Gelsomino L,Giordano C,Panza S,Sisci D,Morelli C,Győrffy B,Bonofiglio D,Andò S,Barone I,Catalano S

MA1-23296 was used in Western Blotting to study exosome contribution in AIR mechanisms, using estrogen-dependent MCF-7 breast cancer cells as models and MCF-7 LTED (Long-Term Estrogen Deprived) subline, modeling AIR.

Fri Aug 14 00:00:00 EDT 2020

Evidence for Enhanced Exosome Production in Aromatase Inhibitor-Resistant Breast Cancer Cells.

International journal of molecular sciences

Augimeri G,La Camera G,Gelsomino L,Giordano C,Panza S,Sisci D,Morelli C,Győrffy B,Bonofiglio D,Andò S,Barone I,Catalano S

Fri Aug 14 00:00:00 EDT 2020

Detection of Viral RNA Fragments in Human iPSC-Cardiomyocytes following Treatment with Extracellular Vesicles from SARS-CoV-2 Coding-Sequence-Overexpressing Lung Epithelial Cells.

bioRxiv : the preprint server for biology

Kwon Y,Nukala SB,Srivastava S,Miyamoto H,Ismail NI,Rehman J,Ong SB,Lee WH,Ong SG

MA1-23296 was used in Western Blotting to demonstrate that SARS-CoV-2 RNA-containing extracellular vesicles represent an indirect route of viral RNA entry into human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).

Wed Jul 01 00:00:00 EDT 2020

Cutaneous T-Cell Lymphoma (CTCL) Cell Line-Derived Extracellular Vesicles Contain HERV-W-Encoded Fusogenic Syncytin-1.

The Journal of investigative dermatology

Laukkanen K,Saarinen M,Mallet F,Aatonen M,Hau A,Ranki A

MA1-23296 was used in Western Blotting to examine the expression and functional properties of syncytin-1 in verified cell lines of the aforementioned forms of CTCLs and also its presence in the extracellular vesicles (EVs) released by the malignant cells.

Wed Jul 01 00:00:00 EDT 2020

Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference.

Communications biology

Trubetckaia O,Lane AE,Qian L,Zhou P,Lane DA

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Mon May 18 00:00:00 EDT 2020

Hypoxia preconditioned renal tubular epithelial cell-derived extracellular vesicles alleviate renal ischaemia-reperfusion injury mediated by the HIF-1α/Rab22 pathway and potentially affected by microRNAs.

International journal of biological sciences

Zhang L,Liu H,Xu K,Ling Z,Huang Y,Hu Q,Lu K,Liu C,Wang Y,Liu N,Zhang X,Xu B,Wu J,Chen S,Zhang G,Chen M

MA1-23296 was used in Western Blotting to demonstrate that HIF-1α/Rab22 pathway mediated RTEC-derived EVs during RIPC.

Mon Apr 06 00:00:00 EDT 2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Nature communications

Dinh PC,Paudel D,Brochu H,Popowski KD,Gracieux MC,Cores J,Huang K,Hensley MT,Harrell E,Vandergriff AC,George AK,Barrio RT,Hu S,Allen TA,Blackburn K,Caranasos TG,Peng X,Schnabel LV,Adler KB,Lobo LJ,Goshe MB,Cheng K

MA1-23296 was used in Western Blotting to show that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.

Fri Feb 28 00:00:00 EST 2020

Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues.

Nature communications

Ñahui Palomino RA,Vanpouille C,Laghi L,Parolin C,Melikov K,Backlund P,Vitali B,Margolis L

MA1-23296 was used in Western Blotting to investigate whether the anti-HIV effect of lactobacilli is mediated by extracellular vesicles released by these bacteria.

Wed Dec 11 00:00:00 EST 2019

Biodistribution of Mesenchymal Stem Cell-Derived Extracellular Vesicles in a Radiation Injury Bone Marrow Murine Model.

International journal of molecular sciences

Wen S,Dooner M,Papa E,Del Tatto M,Pereira M,Borgovan T,Cheng Y,Goldberg L,Liang O,Camussi G,Quesenberry P

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Sat Nov 02 00:00:00 EDT 2019

Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation.

eLife

Stefanius K,Servage K,de Souza Santos M,Gray HF,Toombs JE,Chimalapati S,Kim MS,Malladi VS,Brekken R,Orth K

MA1-23296 was used in Western Blotting to describe a novel molecular understanding of how cancer cell exosomes contribute to cell transformation.

Tue May 28 00:00:00 EDT 2019

Polarized Secretion of Extracellular Vesicles by Mammary Epithelia.

Journal of mammary gland biology and neoplasia

Chin AR,Yan W,Cao M,Liu X,Wang SE

MA1-23296 was used in Western Blotting to explore the potential physiological role of EVs in the mammary gland.

Sat Sep 01 00:00:00 EDT 2018

PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C.

The Journal of biological chemistry

Sharma S,Arunachalam PS,Menon M,Ragupathy V,Satya RV,Jebaraj J,Aralaguppe SG,Rao C,Pal S,Saravanan S,Murugavel KG,Balakrishnan P,Solomon S,Hewlett I,Ranga U

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C.

The Journal of biological chemistry

Sharma S,Arunachalam PS,Menon M,Ragupathy V,Satya RV,Jebaraj J,Aralaguppe SG,Rao C,Pal S,Saravanan S,Murugavel KG,Balakrishnan P,Solomon S,Hewlett I,Ranga U

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C.

The Journal of biological chemistry

Sharma S,Arunachalam PS,Menon M,Ragupathy V,Satya RV,Jebaraj J,Aralaguppe SG,Rao C,Pal S,Saravanan S,Murugavel KG,Balakrishnan P,Solomon S,Hewlett I,Ranga U

Published figure using TSG101 monoclonal antibody (Product # MA1-23296) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility 101; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

Gene Aliases: AI255943; CC2; Rw; TSG10; TSG101; VPS23

UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187

Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088, (Chinese hamster) 100752541

Molecular Function: ubiquitin-protein ligase