|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||External domain of human TSH receptor.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 -1:1000|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:2000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. A suggested positive control for immunohistochemical applications is thyroid gland. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
The thyroid stimulating hormone (thyrotropin) receptor (TSHR) is expressed on the surface of thyrocyte cell membranes and is crucial for thyroid gland growth and function. TSHR and quote;s are targets for the pathogenesis of many autoimmune diseases including Graves and quote; disease and primary myxedema. This 7-transmembrane-domain protein contains 5 potential extracellular N-linked glycosylation sites. Antibodies to TSHR can affect receptor function in a number of different ways. TSHR antibodies which can inhibit TSH binding to the receptor are called TSH binding inhibitor immunoglobulins or TBIIs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.