Immunofluorescent analysis of TUBG1 in centrosomes of fixed NIH-3T3 cells using a TUBG1 polyclonal antibody (Product # PA1-28042) at a dilution of 1:1500 and detected using a Goat anti-Rabbit IgG, Atto448 dye conjugate at a magnification of 100x. Actin was stained using a rhodamine conjugate and nuclei were stained using DAPI.
|Tested species reactivity||Human, Mouse, Sea urchin|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide EEFATEGTDRKDVFFY-K conjugated to KLH, corresponding to amino acids 38-53 of Human gamma Tubulin.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28042 detects TUBG1 from human samples.
PA1-28042 has been successfully used in immunofluorescence, Western blot applications.
The PA1-28042 immunogen is: Synthetic peptide: EEFATEGTDRKDVFFY-K conjugated to KLH, corresponding to amino acids 38-53 of Human gamma Tubulin.
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.