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Product Details
MA5-28605
| Applications | Tested Dilution | Publications |
|---|---|---|
Flow Cytometry (Flow) |
10 µL/1x10^6 cells | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG3, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
CARL-1 |
Immunogen |
human CD255-transfected 2PK-3 cells |
Conjugate |
PE
|
Excitation/Emission Max |
565/576 nm
View spectra
|
Form |
liquid |
Purification |
Size-exclusion chromatography |
Storage buffer |
PBS, pH 7.4, with 0.2% BSA |
Contains |
15mM sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2745564 |
Product Specific Information
The mouse monoclonal antibody CARL-1 recognizes CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to weakly induce apoptosis in many cell types.
Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.
Target Information
TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APO3 ligand; APO3/DR3 ligand; MGC129581; MGC20669; TNF-related WEAK inducer of apoptosis; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; Tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; TWEAK
Gene Aliases: APO3L; DR3LG; TNFSF12; TNLG4A; TWEAK; UNQ181/PRO207
UniProt ID: (Human) O43508
Entrez Gene ID: (Human) 8742
angiogenesis
positive regulation of endothelial cell proliferation
apoptotic process
immune response
signal transduction
cell differentiation
tumor necrosis factor-mediated signaling pathway
endothelial cell migration
positive regulation of protein catabolic process
positive regulation of angiogenesis
extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
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