Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
2-4 µg/mL | - |
ChIP-sequencing (ChIP-Seq) |
4 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Mouse / IgG2a |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Immunogen |
This antibody was raised against a recombinant protein corresponding to full length mouse TWIST1 (twist homolog 1 (Drosophila)). |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl |
Contains |
0.035% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
TWIST1 acts as a transcriptional regulator. It inhibits myogensis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. TWIST also regulates cranial suture patterning and fusion and gene expression differentially depending on dimer composition. Mutations in the gene are implicated in Saethre-Chotzen syndrome, a disease characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-HLH DNA binding protein; bHLHa38; charlie chaplin; Class A basic helix-loop-helix protein 38; H-twist; M-twist; p ACS3; Pluridigite; polydactyly EMS; Skam10Jus; twist basic helix-loop-helix transcription factor 1; twist gene homolog 1; twist homolog 1; TWIST homolog of drosophila; Twist-related protein 1
Gene Aliases:
AA960487; ACS3; BHLHA38; BPES2; BPES3; CRS; CRS1; CSO; M-Twist; Pde; pdt; SCS; Ska10; Ska
UniProt ID: (Human) Q15672, (Mouse) P26687
Entrez Gene ID: (Human) 7291, (Mouse) 22160
Molecular Function:
basic helix-loop-helix transcription factor
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