Immunohistochemical analysis of mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. A TXNL1 polyclonal antibody (Product # PA5-11420) shows strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization and could be abolished with peptide used for immunization (Blocking peptide). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 256-289 amino acids from the C-terminal region of human TXNL1 (TRP32/TrxL)|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse and rat based on sequence homology.
Thioredoxins are a group of small redox active proteins possessing a conserved active site sequence. TrxL (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which shows no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TrxL does not serve as a substrate for thioredoxin reductase in an insulin assay.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.