OriGene

TYR Antibody (UMAB257), UltraMAB™ Mouse Monoclonal

Product Image Gallery

TYR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of cell pellets(The left is SK-MEL-5 and the right is A431) using anti-TYR Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800147)

(1:120°C

TYR Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYR (Tyrosinase) (RC221797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TYR (Tyrosinase). (1:2000)

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:1200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

UMAB257

Immunogen

Human recombinant protein fragment corresponding to amino acids 19-320 of human TYR produced in E.coli.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATN; CMM8; LB24-AB; Monophenol monooxygenase; OCA1; OCA1A; OCAIA; oculocutaneous albinism IA; SHEP3; SK29-AB; Tumor rejection antigen AB; Tyrosinase

Gene Aliases: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3; TYR

UniProt ID: (Human) P14679

Entrez Gene ID: (Human) 7299

Function(s)

melanin biosynthetic process from tyrosine eye pigment biosynthetic process visual perception cell proliferation response to UV response to vitamin D melanin biosynthetic process thymus development response to cAMP oxidation-reduction process

Process(es)

monooxygenase activity monophenol monooxygenase activity copper ion binding protein binding protein homodimerization activity protein heterodimerization activity

TYR Antibody (UMAB257), UltraMAB™ Mouse Monoclonal

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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