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Talin 1 Antibody (MA5-49244) in WB
Western blot analysis of TLN1 using TLN1 antibody (Product # MA5-49244). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Raji whole cell lysates; Lane 2: human SW620 whole cell lysates; Lane 3: human COLO320 whole cell lysates; Lane 4: human 293T whole cell lysates; Lane 5: rat lung tissue lysates; Lane 6: mouse lung tissue lysates; Lane 7: mouse liver tissue lysates. After e... View More
Product Details
MA5-49244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2G10D2
Immunogen
E.coli-derived human Talin 1/TLN1 recombinant protein (Position: Q1658-N1876).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074902
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Talin-1
Gene Aliases: ILWEQ; KIAA1027; TLN; TLN1
UniProt ID: (Human) Q9Y490, (Mouse) P26039
Entrez Gene ID: (Human) 7094, (Rat) 313494, (Mouse) 21894
cytoskeletal anchoring at plasma membrane
cell-substrate junction assembly
biological_process
cortical actin cytoskeleton organization
platelet aggregation
cell adhesion
platelet degranulation
movement of cell or subcellular component
muscle contraction
cell-cell junction assembly
viral process
IRE1-mediated unfolded protein response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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