|Tested species reactivity||Bovine, Human|
|Published species reactivity||Non-human primate, Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MN1000B targets Tau in ELISA, WB and IHC applications and shows reactivity with Bovine, and Human samples.
The MN1000B immunogen is purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.
MN1000B detects Tau which has a predicted molecular weight of approximately 79 kDa.
Paired helical filament (PHF) is a major component of the neurofibrillary tangles involved in the pathology of Alzheimer and quote;s disease. PHFs are composed of the microtubule-associated protein tau in a hyper-phosphorylated state (ref1). Tau protein is produced by a single gene expressed predominantly in neurons. The Tau gene undergoes complex alternative splicing, yielding six different isoforms of tau in the adult brain. Following translation, the tau protein can be further modified by phosphorylation at several different sites
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes.
MN1000B was used in immunohistochemistry - free floating, immunohistochemistry - paraffin section, and western blot to identify NFE2L2/NRF2 as a regulator of autophagy gene expression and study its role in Alzheimer disease
|Pajares M,Jiménez-Moreno N,García-Yagüe ÁJ,Escoll M,de Ceballos ML,Van Leuven F,Rábano A,Yamamoto M,Rojo AI,Cuadrado A||Autophagy (12:1902)||2016|
Sleep deprivation impairs memory, tau metabolism, and synaptic integrity of a mouse model of Alzheimer's disease with plaques and tangles.
MN1000B was used in western blot to study the deleterious effects of sleep deprivation on memory and Tau pathology in a murine Alzheimer's disease model
|Di Meco A,Joshi YB,Praticò D||Neurobiology of aging (35:1813)||2014|
Mammalian target of rapamycin hyperactivity mediates the detrimental effects of a high sucrose diet on Alzheimer's disease pathology.
MN1000B was used in western blot to study the role of mTOR hyperactivation in the mechanism by which a high-sucrose diet promotes beta-amyloid pathology in Alzheimer's disease
|Orr ME,Salinas A,Buffenstein R,Oddo S||Neurobiology of aging (35:1233)||2014|
Premature lethality, hyperactivity, and aberrant phosphorylation in transgenic mice expressing a constitutively active form of Fyn.
MN1000B was used in western blot to study the effects of transgenically expressing a constitutively active Y531F Fyn mutant on murine life-span, activity and protein phosphorylation
|Xia D,Götz J||Frontiers in molecular neuroscience (7:null)||2014|
Suppression of InsP3 receptor-mediated Ca2+ signaling alleviates mutant presenilin-linked familial Alzheimer's disease pathogenesis.
MN1000B was used in western blot to study the role of exagerated Ca(2+) signaling mediated by InsP3 receptor1 in the pathogenesis of mutant presenilin-linked Alzheimer's disease
|Shilling D,Müller M,Takano H,Mak DO,Abel T,Coulter DA,Foskett JK||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:6910)||2014|
Annonacin, a natural lipophilic mitochondrial complex I inhibitor, increases phosphorylation of tau in the brain of FTDP-17 transgenic mice.
MN1000B was used in western blot to study the increased tau phosphorylation following treatment with a mitochondrial complex I inhibitor in a tau transgenic mouse model
|Yamada ES,Respondek G,Müssner S,de Andrade A,Höllerhage M,Depienne C,Rastetter A,Tarze A,Friguet B,Salama M,Champy P,Oertel WH,Höglinger GU||Experimental neurology (253:113)||2014|
Endogenous murine tau promotes neurofibrillary tangles in 3xTg-AD mice without affecting cognition.
MN1000B was used in western blot to study the effect of endogenous murine tau on cognition and neurofibrillary tangles in a murine Alzheimer's disease model transgenically expressing human tau
|Baglietto-Vargas D,Kitazawa M,Le EJ,Estrada-Hernandez T,Rodriguez-Ortiz CJ,Medeiros R,Green KN,LaFerla FM||Neurobiology of disease (62:407)||2014|
Mifepristone alters amyloid precursor protein processing to preclude amyloid beta and also reduces tau pathology.
MN1000B was used in western blot to study the reduced alpha- and beta-cleavage of APP and reduced phosporylated tau accumulation in transgenic mice treated with mifepristone
|Baglietto-Vargas D,Medeiros R,Martinez-Coria H,LaFerla FM,Green KN||Biological psychiatry (74:357)||2013|
Fractalkine overexpression suppresses tau pathology in a mouse model of tauopathy.
MN1000B was used in western blot to study the beneficial effects on tau pathology in a murine transgenic tauopathy model of the virally-mediated overexpression of soluble fractalkine
|Nash KR,Lee DC,Hunt JB,Morganti JM,Selenica ML,Moran P,Reid P,Brownlow M,Guang-Yu Yang C,Savalia M,Gemma C,Bickford PC,Gordon MN,Morgan D||Neurobiology of aging (34:1540)||2013|
Active glycogen synthase kinase-3 and tau pathology-related tyrosine phosphorylation in pR5 human tau transgenic mice.
MN1000B was used in western blot to study the association of GSK3 activity with tau phosphorylation and neuronal tyrosine phosphorylation in a murine model expressing human P301L tau
|Köhler C,Dinekov M,Götz J||Neurobiology of aging (34:1369)||2013|
PINCH in the cellular stress response to tau-hyperphosphorylation.
MN1000B was used in western blot to study the ability of PINCH in bind and stabilize hyperphoshorylated tau
|Ozdemir AY,Rom I,Kovalevich J,Yen W,Adiga R,Dave RS,Langford D||PloS one (8:null)||2013|
Clinical, neuropathological, and biochemical characterization of the novel tau mutation P332S.
MN1000B was used in western blot to study the clinical and molecular characteristics of a novel P332S tau mutation
|Deramecourt V,Lebert F,Maurage CA,Fernandez-Gomez FJ,Dujardin S,Colin M,Sergeant N,Buée-Scherrer V,Clot F,Ber IL,Brice A,Pasquier F,Buée L||Journal of Alzheimer's disease : JAD (31:741)||2012|
Calpain inhibitor A-705253 mitigates Alzheimer's disease-like pathology and cognitive decline in aged 3xTgAD mice.
MN1000B was used in western blot to study the beneficial effects of a novel calpain inhibitor on Alzheimers's disease pathology and cognitive decline in transgenic mouse model
|Medeiros R,Kitazawa M,Chabrier MA,Cheng D,Baglietto-Vargas D,Kling A,Moeller A,Green KN,LaFerla FM||The American journal of pathology (181:616)||2012|
|Non-human primate||Not Cited||
Membrane-microdomain localization of amyloid ß-precursor protein (APP) C-terminal fragments is regulated by phosphorylation of the cytoplasmic Thr668 residue.
MN1000B was used in western blot to study the role of phosphorylation in the membrane-microdomain localization of amyloid ?-precursor protein C-terminal fragments
|Matsushima T,Saito Y,Elliott JI,Iijima-Ando K,Nishimura M,Kimura N,Hata S,Yamamoto T,Nakaya T,Suzuki T||The Journal of biological chemistry (287:19715)||2012|
Hippocampal BDNF expression in a tau transgenic mouse model.
MN1000B was used in western blot to study whether tau pathology is associated with BDNF downregulation in a transgenic mouse model
|Burnouf S,Belarbi K,Troquier L,Derisbourg M,Demeyer D,Leboucher A,Laurent C,Hamdane M,Buee L,Blum D||Current Alzheimer research (9:406)||2012|
Altered expression of brain acetylcholinesterase in FTDP-17 human tau transgenic mice.
MN1000B was used in western blot to identify the relationship between acetylcholinesterase and P-tau expression in neurodegeneration
|Silveyra MX,García-Ayllón MS,de Barreda EG,Small DH,Martínez S,Avila J,Sáez-Valero J||Neurobiology of aging (33:624.e23)||2012|
Prolonged nitric oxide treatment induces tau aggregation in SH-SY5Y cells.
MN1000B was used in western blot to investigate the effect of prolonged NO treatment on the aggregation of tau in SH-SY5Y cells
|Takahashi M,Chin Y,Nonaka T,Hasegawa M,Watanabe N,Arai T||Neuroscience letters (510:48)||2012|
LRRK2 phosphorylates tubulin-associated tau but not the free molecule: LRRK2-mediated regulation of the tau-tubulin association and neurite outgrowth.
MN1000B was used in western blot to investigate the important roles of LRRK2 in phosphorylation-mediated dissociation of tau from microtubules
|Kawakami F,Yabata T,Ohta E,Maekawa T,Shimada N,Suzuki M,Maruyama H,Ichikawa T,Obata F||PloS one (7:null)||2012|
Wild type and P301L mutant Tau promote neuro-inflammation and ¿-Synuclein accumulation in lentiviral gene delivery models.
MN1000B was used in western blot to investigate the effects of wild type and P301L mutant tau on neuro-inflammation and alpha-synuclein accumulation in a rat model
|Khandelwal PJ,Dumanis SB,Herman AM,Rebeck GW,Moussa CE||Molecular and cellular neurosciences (49:44)||2012|
Loss of muscarinic M1 receptor exacerbates Alzheimer's disease-like pathology and cognitive decline.
MN1000B was used in western blot to study the deleterious effects of muscarinic M1 receptor knockout on Alzheimer's disease pathology and cognitive decline in a transgenic mouse model
|Medeiros R,Kitazawa M,Caccamo A,Baglietto-Vargas D,Estrada-Hernandez T,Cribbs DH,Fisher A,LaFerla FM||The American journal of pathology (179:980)||2011|
Long term changes in phospho-APP and tau aggregation in the 3xTg-AD mice following cerebral ischemia.
MN1000B was used in western blot to investigate the phosphorylation and aggregation of tau protein in the 3xTg-AD mice
|Koike MA,Garcia FG,Kitazawa M,Green KN,Laferla FM||Neuroscience letters (495:55)||2011|
Hippocampal tauopathy in tau transgenic mice coincides with impaired hippocampus-dependent learning and memory, and attenuated late-phase long-term depression of synaptic transmission.
MN1000B was used in western blot to investigate the influence of hippocampal tauopathy in learning, memory and neural functions in transgenic mice
|Van der Jeugd A,Ahmed T,Burnouf S,Belarbi K,Hamdame M,Grosjean ME,Humez S,Balschun D,Blum D,Buée L,D'Hooge R||Neurobiology of learning and memory (95:296)||2011|
Gateway-compatible lentiviral transfer vectors for ubiquitin promoter driven expression of fluorescent fusion proteins.
MN1000B was used in western blot to evaluate the transfection vector for fluorescent fusion protei expression in 293T and SH-SY5Y cells
|Krupka N,Strappe P,Götz J,Ittner LM||Plasmid (63:155)||2010|
GLP-1 receptor stimulation reduces amyloid-beta peptide accumulation and cytotoxicity in cellular and animal models of Alzheimer's disease.
MN1000B was used in western blot to investigate the effect of GLP-1 receptor stimulation on amyloid-beta peptide accumulation and cytotoxicity in Alzheimer disease model
|Li Y,Duffy KB,Ottinger MA,Ray B,Bailey JA,Holloway HW,Tweedie D,Perry T,Mattson MP,Kapogiannis D,Sambamurti K,Lahiri DK,Greig NH||Journal of Alzheimer's disease : JAD (19:1205)||2010|
Memantine improves cognition and reduces Alzheimer's-like neuropathology in transgenic mice.
MN1000B was used in western blot to investigate the effect of memantine on Alzheimer disease treatment in mice
|Martinez-Coria H,Green KN,Billings LM,Kitazawa M,Albrecht M,Rammes G,Parsons CG,Gupta S,Banerjee P,LaFerla FM||The American journal of pathology (176:870)||2010|
A cellular model to monitor proteasome dysfunction by alpha-synuclein.
MN1000B was used in western blot to study the effect of alpha-synuclein on proteasome function
|Nonaka T,Hasegawa M||Biochemistry (48:8014)||2009|
Biochemical characterization of Abeta and tau pathologies in mild cognitive impairment and Alzheimer's disease.
MN1000B was used in western blot to quantify the abeta and tau pathologies in mild cognitive impairment and Alzheimer disease
|Tremblay C,Pilote M,Phivilay A,Emond V,Bennett DA,Calon F||Journal of Alzheimer's disease : JAD (12:377)||2007|
Age-related downregulation of the CaV3.1 T-type calcium channel as a mediator of amyloid beta production.
MN1000B was used in immunohistochemistry and western blot to study the potential mechanism by which the age-related decline in CaV3.1 T-type calcium channel expression leads to increased production of beta-amyloid
|Rice RA,Berchtold NC,Cotman CW,Green KN||Neurobiology of aging (35:1002)||2014|
Synergistic effects of amyloid-beta and wild-type human tau on dendritic spine loss in a floxed double transgenic model of Alzheimer's disease.
MN1000B was used in immunohistochemistry and western blot to study dendritic spine loss in a transgenic murine model of Alzheimer's disease and the synergistic effects of Abeta and tau
|Chabrier MA,Cheng D,Castello NA,Green KN,LaFerla FM||Neurobiology of disease (64:107)||2014|
Increased expression of the receptor for advanced glycation end products in neurons and astrocytes in a triple transgenic mouse model of Alzheimer's disease.
MN1000B was used in immunohistochemistry to study the elevated neuronal and astrocyte expression of RAGE in a murine transgenic model of Alzheimer's disease
|Choi BR,Cho WH,Kim J,Lee HJ,Chung C,Jeon WK,Han JS||Experimental and molecular medicine (46:null)||2014|
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
MN1000B was used in immunohistochemistry, immunoprecipitation, and western blot to study the beneficial effects of increasing the O-GlucNAcylation of brain proteins on the survival and breathing of aged tau P301L transgenic mice
|Borghgraef P,Menuet C,Theunis C,Louis JV,Devijver H,Maurin H,Smet-Nocca C,Lippens G,Hilaire G,Gijsen H,Moechars D,Van Leuven F||PloS one (8:null)||2013|
Impaired plasticity of cortical dendritic spines in P301S tau transgenic mice.
MN1000B was used in immunohistochemistry to study the potential involvement of pre-fibrilliary tau in the defective plasticity of cortical dendritic spines observed in transgenic mice bearing the P301S tau mutation
|Hoffmann NA,Dorostkar MM,Blumenstock S,Goedert M,Herms J||Acta neuropathologica communications (1:null)||2013|
Protection of primary neurons and mouse brain from Alzheimer's pathology by molecular tweezers.
MN1000B was used in immunohistochemistry to study the ability of lysine-specific molecular tweezers to protect against Alzheimer's disease pathology
|Attar A,Ripoli C,Riccardi E,Maiti P,Li Puma DD,Liu T,Hayes J,Jones MR,Lichti-Kaiser K,Yang F,Gale GD,Tseng CH,Tan M,Xie CW,Straudinger JL,Klärner FG,Schrader T,Frautschy SA,Grassi C,Bitan G||Brain : a journal of neurology (135:3735)||2012|
Human P301L-mutant tau expression in mouse entorhinal-hippocampal network causes tau aggregation and presynaptic pathology but no cognitive deficits.
MN1000B was used in immunohistochemistry to study whether tau pathology caused by overexpression of mutant P301L tau in the murine entorhinal-hippocampal network leads to cognitive defects
|Harris JA,Koyama A,Maeda S,Ho K,Devidze N,Dubal DB,Yu GQ,Masliah E,Mucke L||PloS one (7:null)||2012|
Effects of the superoxide dismutase/catalase mimetic EUK-207 in a mouse model of Alzheimer's disease: protection against and interruption of progression of amyloid and tau pathology and cognitive decline.
MN1000B was used in immunohistochemistry to study the role of oxidative stress in Alzheimer's disease pathogenesis and progression
|Clausen A,Xu X,Bi X,Baudry M||Journal of Alzheimer's disease : JAD (30:183)||2012|
Frontal cortex neuropathology in dementia pugilistica.
MN1000B was used in immunohistochemistry to investigate the neuropathological changes in dementia pugilistica patients
|Saing T,Dick M,Nelson PT,Kim RC,Cribbs DH,Head E||Journal of neurotrauma (29:1054)||2012|
Morin attenuates tau hyperphosphorylation by inhibiting GSK3ß.
MN1000B was used in immunohistochemistry to study the ability of the GSKbeta inhibitor morin to reduce tau hyperphosphorylation
|Gong EJ,Park HR,Kim ME,Piao S,Lee E,Jo DG,Chung HY,Ha NC,Mattson MP,Lee J||Neurobiology of disease (44:223)||2011|
A peculiar constellation of tau pathology defines a subset of dementia in the elderly.
MN1000B was used in immunohistochemistry to investigate the changes of tau pattern in some older patients with dementia
|Kovacs GG,Molnár K,László L,Ströbel T,Botond G,Hönigschnabl S,Reiner-Concin A,Palkovits M,Fischer P,Budka H||Acta neuropathologica (122:205)||2011|
Controlled cortical impact traumatic brain injury in 3xTg-AD mice causes acute intra-axonal amyloid-ß accumulation and independently accelerates the development of tau abnormalities.
MN1000B was used in immunohistochemistry to investigate the effect of traumatic brain injury on the development of Alzheimer disease pathology
|Tran HT,LaFerla FM,Holtzman DM,Brody DL||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:9513)||2011|
Tau transgenic mice as models for cerebrospinal fluid tau biomarkers.
MN1000B was used in immunohistochemistry to detect Tau protein in cerebrospinal fluid
|Barten DM,Cadelina GW,Hoque N,DeCarr LB,Guss VL,Yang L,Sankaranarayanan S,Wes PD,Flynn ME,Meredith JE,Ahlijanian MK,Albright CF||Journal of Alzheimer's disease : JAD (24 Suppl 2:127)||2011|
Multiple events lead to dendritic spine loss in triple transgenic Alzheimer's disease mice.
MN1000B was used in immunohistochemistry to investigate the dendritic spine loss during the progression of Alzheimer disease in a mouse model
|Bittner T,Fuhrmann M,Burgold S,Ochs SM,Hoffmann N,Mitteregger G,Kretzschmar H,LaFerla FM,Herms J||PloS one (5:null)||2010|
Spatially pathogenic forms of tau detected in Alzheimer's disease brain tissue by fluorescence lifetime-based Förster resonance energy transfer.
MN1000B was used in immunohistochemistry to develop a FRET-based method to detect pathogenic forms of tau.
|Larionov S,Wielgat P,Wang Y,Thal DR,Neumann H||Journal of neuroscience methods (192:127)||2010|
Early oligodendrocyte/myelin pathology in Alzheimer's disease mice constitutes a novel therapeutic target.
MN1000B was used in immunohistochemistry to investigate the effect of Abeta(1-42) on the survival of mOP cells in Alzheimer disease mouse model
|Desai MK,Mastrangelo MA,Ryan DA,Sudol KL,Narrow WC,Bowers WJ||The American journal of pathology (177:1422)||2010|
Detailed immunohistochemical characterization of temporal and spatial progression of Alzheimer's disease-related pathologies in male triple-transgenic mice.
MN1000B was used in immunohistochemistry to study the temporal and spatial progression of AD-like pathology in male 3xTg-AD mice
|Mastrangelo MA,Bowers WJ||BMC neuroscience (9:null)||2008|
Passive immunization with Tau oligomer monoclonal antibody reverses tauopathy phenotypes without affecting hyperphosphorylated neurofibrillary tangles.
MN1000B was used in ELISA and immunohistochemistry to study the ability of an anti-oligomeric tau monoclonal antibody to specifically reduce oligomeric tau and improve memory and locomotion in a murine Alzheimer's disease model
|Castillo-Carranza DL,Sengupta U,Guerrero-Muñoz MJ,Lasagna-Reeves CA,Gerson JE,Singh G,Estes DM,Barrett AD,Dineley KT,Jackson GR,Kayed R||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:4260)||2014|
Tau-tubulin kinase 1 expression, phosphorylation and co-localization with phospho-Ser422 tau in the Alzheimer's disease brain.
MN1000B was used in ELISA to investigate the characteristics of TTBK1 in the brain of AD patients
|Lund H,Cowburn RF,Gustafsson E,Strömberg K,Svensson A,Dahllund L,Malinowsky D,Sunnemark D||Brain pathology (Zurich, Switzerland) (23:378)||2013|
The microtubule-stabilizing agent, epothilone D, reduces axonal dysfunction, neurotoxicity, cognitive deficits, and Alzheimer-like pathology in an interventional study with aged tau transgenic mice.
MN1000B was used in ELISA to study mechaisms underlying the therapeutic effects of epothilone D in a murine transgenic tau model and the significance for Alzheimer's disease
|Zhang B,Carroll J,Trojanowski JQ,Yao Y,Iba M,Potuzak JS,Hogan AM,Xie SX,Ballatore C,Smith AB,Lee VM,Brunden KR||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:3601)||2012|
Tau in cerebrospinal fluid: a sensitive sandwich enzyme-linked immunosorbent assay using tyramide signal amplification.
MN1000B was used in ELISA to develop a novel ELISA assay for quantification of tau in cerebrospinal fluid
|Yamamori H,Khatoon S,Grundke-Iqbal I,Blennow K,Ewers M,Hampel H,Iqbal K||Neuroscience letters (418:186)||2007|
Impact of N-tau on adult hippocampal neurogenesis, anxiety, and memory.
MN1000B was used in immunocytochemistry and immunohistochemistry to study the effects of a pathogenic tau fragment on hippocampal neurogenesis, behaviour and learning
|Pristerà A,Saraulli D,Farioli-Vecchioli S,Strimpakos G,Costanzi M,di Certo MG,Cannas S,Ciotti MT,Tirone F,Mattei E,Cestari V,Canu N||Neurobiology of aging (34:2551)||2013|
6-amino-4-(pyrimidin-4-yl)pyridones: novel glycogen synthase kinase-3ß inhibitors.
MN1000B was used in immunoprecipitation to identify and characterize a novel class of GSK3 beta inhibitors
|Coffman K,Brodney M,Cook J,Lanyon L,Pandit J,Sakya S,Schachter J,Tseng-Lovering E,Wessel M||Bioorganic and medicinal chemistry letters (21:1429)||2011|