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Immunofluorescence analysis of Tau was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau Mouse Monoclonal Antibody (136400) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human , Rabbit , Mouse , Rat , Cattle , Chicken , Clawed frog|
|Published species reactivity||Primate , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tamoxifen inhibits CDK5 kinase activity by interacting with p35/p25 and modulates the pattern of tau phosphorylation.
13-6400 was used in western blot to identify small-molecules that inhibit the interaction between CDK5 and p25
|Corbel C,Zhang B,Le Parc A,Baratte B,Colas P,Couturier C,Kosik KS,Landrieu I,Le Tilly V,Bach S||Chemistry & biology (22:472)||2015|
Neurodegeneration in heterozygous Niemann-Pick type C1 (NPC1) mouse: implication of heterozygous NPC1 mutations being a risk for tauopathy.
||Yu W,Ko M,Yanagisawa K,Michikawa M||The Journal of biological chemistry (280:27296)||2005|
Fyn phosphorylates human MAP-2c on tyrosine 67.
||Zamora-Leon SP,Bresnick A,Backer JM,Shafit-Zagardo B||The Journal of biological chemistry (280:1962)||2005|
Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17.
||Clark LN,Poorkaj P,Wszolek Z,Geschwind DH,Nasreddine ZS,Miller B,Li D,Payami H,Awert F,Markopoulou K,Andreadis A,D'Souza I,Lee VM,Reed L,Trojanowski JQ,Zhukareva V,Bird T,Schellenberg G,Wilhelmsen KC||Proceedings of the National Academy of Sciences of the United States of America (95:13103)||1998|
The neuropathology of a chromosome 17-linked autosomal dominant parkinsonism and dementia ("pallido-ponto-nigral degeneration").
||Reed LA,Schmidt ML,Wszolek ZK,Balin BJ,Soontornniyomkij V,Lee VM,Trojanowski JQ,Schelper RL||Journal of neuropathology and experimental neurology (57:588)||1998|
Total body 100-mGy X-irradiation does not induce Alzheimer's disease-like pathogenesis or memory impairment in mice.
||Wang B,Tanaka K,Ji B,Ono M,Fang Y,Ninomiya Y,Maruyama K,Izumi-Nakajima N,Begum N,Higuchi M,Fujimori A,Uehara Y,Nakajima T,Suhara T,Ono T,Nenoi M||Journal of radiation research (55:84)||2014|
Multiple Eph receptors and B-class ephrins regulate midline crossing of corpus callosum fibers in the developing mouse forebrain.
||Mendes SW,Henkemeyer M,Liebl DJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:882)||2006|
MTBT2, BOS_19251, MTBT1, TAU, DDPAC, AW045860, RNPTAU, AI413597, Tau, PPND, Mtapt, tau, FTDP-17, pTau, MAPTL, MSTD
G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, neurofibrillary tangle protein, paired helical filament-tau, Tau microtubule-associated protein, microtubule associated protein tau, MAPT, Microtubule-associated protein tau, MTBT1