Immunofluorescence analysis of Tau (Cleavage site 421 / 422) Antibody (TauC3) was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau (Cleavage site 421 / 422) Antibody (TauC3) (AHB0061) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||KLH-CSSTGSIDMVD, corresponding to the C terminus of tau truncated at Asp421.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments.
AHB0061 was used in immunohistochemistry - paraffin section to study the relationship between neurofibrillary tangles and raft domains
|Nishikawa T,Takahashi T,Nakamori M,Hosomi N,Maruyama H,Miyazaki Y,Izumi Y,Matsumoto M||Neuropathology and applied neurobiology (42:639)||2016|
Carboxy terminus heat shock protein 70 interacting protein reduces tau-associated degenerative changes.
AHB0061 was used in western blot to test if CHIP ameliorates the pathological changes associated with tau aggregation.
|Saidi LJ,Polydoro M,Kay KR,Sanchez L,Mandelkow EM,Hyman BT,Spires-Jones TL||Journal of Alzheimer's disease : JAD (44:937)||2015|
||Pericyte loss influences Alzheimer-like neurodegeneration in mice.||Sagare AP,Bell RD,Zhao Z,Ma Q,Winkler EA,Ramanathan A,Zlokovic BV||Nature communications (4:null)||2013|
|Mouse||Not Cited||Pericyte loss influences Alzheimer-like neurodegeneration in mice.||Sagare AP,Bell RD,Zhao Z,Ma Q,Winkler EA,Ramanathan A,Zlokovic BV||Nature communications (4:null)||2013|