|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The entire region of the Tfg2p protein (amino acid residues 1-400)|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4944 detects the Tfg2p subunit of TFIIF in budding yeast samples.
PA1-4944 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~54 kDa protein (Tfg2p) in yeast samples.
PA1-4944 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire region of the Tfg2p protein (amino acid residues 1-400).
The basal transcription factor TFIIF binds with RNA polymerase II and takes part in the transcription initiation reaction and the elongation reaction. The budding yeast TFIIF is composed of three subunits, Tfg1p (735aa), Tfg2p (400aa) and Taf14p (244aa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.