|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human thioredoxin 1 protein expressed in E. coli.|
|Storage buffer||HEPES with 50% glycerol, 0.15M NaCl, 0.01% BSA|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 ug|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
A suggested positive control for this product is HeLa cells.
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cystein residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53 , NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Long-term ursodeoxycholate improves circulating redox changes in primary biliary cirrhotic patients.
LF-MA0077 was used in ELISA to investigate the influence of ursodeoxycholate treatment on circulating redox changes in patients with primary biliary cirrhosis
|Grattagliano I,Palmieri VO,Portincasa P,Minerva F,Palasciano G||Clinical biochemistry (44:1400)||2011|