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|Tested species reactivity||Human|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, recombinant, human thioredoxin reductase 1 protein expressed in E. coli.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.01% BSA, 50% glycerol, 0.15M NaCl|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||1-2 ul|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control for this product is HeLa cells.
The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxidoreductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers.
LF-MA0020 was used in western blot to study how hsc70 reduces the stress response in Xenopus oocytes
|Mifflin LC,Cohen RE||The Journal of biological chemistry (269:15718)||1994|
gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; KM-102-derived reductase-like factor; oxidoreductase; testis tissue sperm-binding protein Li 46a; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; trxR1
GRIM-12; GRIM12; KDRF; TR; TR1; TRXR1; TXNR; TXNRD1