Immunofluorescent analysis of Thioredoxin Reductase 1 showing staining in the nucleus of MCF-7 cells. MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Thioredoxin Reductase 1 polyclonal antibody (Product # PA5-34685) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 69 and 313 of TrxR1 (Uniprot ID#Q16881)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34685 targets Thioredoxin Reductase 1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-34685 immunogen is recombinant fragment corresponding to a region within amino acids 69 and 313 of TrxR1 (Uniprot ID#Q16881).
This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
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