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Immunofluorescent analysis of Thioredoxin Reductase 1 showing staining in the nucleus of MCF-7 cells. MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Thioredoxin Reductase 1 polyclonal antibody (Product # PA5-34685) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 69 and 313 of TrxR1 (Uniprot ID#Q16881)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34685 targets Thioredoxin Reductase 1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-34685 immunogen is recombinant fragment corresponding to a region within amino acids 69 and 313 of TrxR1 (Uniprot ID#Q16881).
This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; oxidoreductase; testis tissue sperm-binding protein Li 46a; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; TR1; TRXR1; TXNR
GRIM-12; GRIM12; KDRF; TR; TR1; TRXR1; TXNR; TXNRD1