HYB 109-03-02 is specific for human thrombin. The antibody reacts with hirudin, a direct inhibitor of thrombin. No cross-reaction is seen with pro-thrombin.
HYB 109-03-02 has been successfully used in ELISA and Western blot procedures. HYB 109-03-02 reacts strongly with thrombin. Strong reaction is seen in ELISA with thrombin directly coated onto the microtiter well. In Western blotting after SDS-PAGE, HYB 109-03-02 reacts with thrombin in both reduced and non-reduced forms. The antigenicity of thrombin is lost by biotinylation.
The HYB 109-03-02 immunogen is thrombin isolated from human serum.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is ~37 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activation peptide fragment 1; Activation peptide fragment 2; Coagulation factor II; coagulation factor II (thrombin); Coagulation factor II (Thrombin) receptor-like 1; prepro-coagulation factor II; Prothombin; Prothrombin; prothrombin B-chain; serine protease; Thrombin heavy chain; Thrombin light chain
Gene Aliases: F2; PT; RPRGL2; THPH1
UniProt ID: (Human) P00734
Entrez Gene ID: (Human) 2147