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Immunofluorescent analysis of CD141 (green) showing staining in the membrane of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD141 monoclonal antibody (Product # MA5-11454) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein encoding the six repeated EGF domains of human thrombomodulin|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:2|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11454 targets CD141 in IHC (P), FACS and ICC/IF applications and shows reactivity with Human and Rat samples.
The MA5-11454 immunogen is recombinant protein encoding the six repeated EGF domains of human thrombomodulin.
Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The endothelial microenvironment in the venous valvular sinus: thromboresistance trends and inter-individual variation.
MA5-11454 was used in immunohistochemistry to examine the gene expression level in different regions of valve sinus
|Trotman WE,Taatjes DJ,Callas PW,Bovill EG||Histochemistry and cell biology (135:141)||2011|
The urokinase receptor supports tumorigenesis of human malignant pleural mesothelioma cells.
MA5-11454 was used in immunohistochemistry to investigate the role of urokinase receptor in the pathogenesis of malignant pleural mesothelioma
|Tucker TA,Dean C,Komissarov AA,Koenig K,Mazar AP,Pendurthi U,Allen T,Idell S||American journal of respiratory cell and molecular biology (42:685)||2010|
Valves of the deep venous system: an overlooked risk factor.
MA5-11454 was used in immunohistochemistry to examine the rolo of valvular sinus endothelium in thrombosis
|Brooks EG,Trotman W,Wadsworth MP,Taatjes DJ,Evans MF,Ittleman FP,Callas PW,Esmon CT,Bovill EG||Blood (114:1276)||2009|
Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats.
MA5-11454 was used in immunohistochemistry to study the potential pathogenic mechanisms underlying interstitial fibrosis after renal ablation in rats
|Zhang B,Liang X,Shi W,Ye Z,He C,Hu X,Liu S||Nephrology (Carlton, Vic.) (10:351)||2005|
Confocal laser scanning microscope study of cytokeratin immunofluorescence differences between villous and extravillous trophoblast: cytokeratin downregulation in pre-eclampsia.
MA5-11454 was used in immunohistochemistry to study differences in cytokeratin levels between villous and extravillous trophoblasts using confocal laser scanning microscopy
|Ockleford CD,Smith RK,Byrne S,Sanders R,Bosio P||Microscopy research and technique (64:43)||2004|
Cellular regulation of blood coagulation: a model for venous stasis.
MA5-11454 was used in immunocytochemistry to develop a model system to study venous stasis.
|Campbell JE,Brummel-Ziedins KE,Butenas S,Mann KG||Blood (116:6082)||2010|
Cell fusion-independent differentiation of neural stem cells to the endothelial lineage.
MA5-11454 was used in immunocytochemistry to investigate the stem-cell plasticity of mouse neural stem cells
|Wurmser AE,Nakashima K,Summers RG,Toni N,D'Amour KA,Lie DC,Gage FH||Nature (430:350)||2004|
AHUS6; BDCA3; CD141; CD141 antigen; DC141; fetomodulin; THBD; THPH12; THRM; thrombomdulin; thrombomodulin
AHUS6; BDCA3; CD141; THBD; THPH12; THRM; TM