|Tested species reactivity||Human, Rabbit|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human endothelium (thrombomodulin)|
|Storage buffer||tissue culture supernatant diluted in 0.2M tris HCl, pH 7.4, with 5-10% FBS|
|Contains||0.01% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
|Immunohistochemistry (Frozen) (IHC (F))||1:10 -1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10 -1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is lymph node. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.