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Thrombopoietin Antibody (PA5-80125) in IHC (P)
Immunohistochemistry analysis of TPO on paraffin-embedded mouse kidney tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TPO polyclonal antibody (Product# PA5-80125) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development... View More
Product Details
PA5-80125
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747239
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse liver tissue. IHC: mouse kidney tissue, mouse spleen tissue, rat kidney tissue, mouse liver tissue, rat spleen tissue.
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; ML; Myeloproliferative leukemia virus oncogene ligand; Thrombopoietin
Gene Aliases: Mgdf; Ml; Mpllg; Thpo; Tpo
UniProt ID: (Rat) P49745, (Mouse) P40226
Entrez Gene ID: (Rat) 81811, (Mouse) 21832
positive regulation of protein phosphorylation
cell proliferation
positive regulation of cell proliferation
myeloid cell differentiation
response to lipopolysaccharide
thrombopoietin-mediated signaling pathway
positive regulation of megakaryocyte differentiation
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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