|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Full-length purified human, mouse, and rat hrombospondin 1 derived from the supernatant of thrombin-activated platelets|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIF-2¿-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction.
39-9300 was used in immunocytochemistry and western blot to investigate if hypoxia regulates TSP1 in the lung to contribute to pulmonary arterial hypertension
|Labrousse-Arias D,Castillo-González R,Rogers NM,Torres-Capelli M,Barreira B,Aragonés J,Cogolludo Á,Isenberg JS,Calzada MJ||Cardiovascular research (109:115)||2016|
Effect of LSKL peptide on thrombospondin 1-mediated transforming growth factor ß signal activation and liver regeneration after hepatectomy in an experimental model.
39-9300 was used in immunohistochemistry - paraffin section to test if a leucine-serine-lysine-leucine peptide promotes liver regeneration.
|Kuroki H,Hayashi H,Nakagawa S,Sakamoto K,Higashi T,Nitta H,Hashimoto D,Chikamoto A,Beppu T,Baba H||The British journal of surgery (102:813)||2015|
Blunted muscle angiogenic training-response in COPD patients versus sedentary controls.
39-9300 was used in western blot to compare muscle functional responses and angio-adaptations after training in chronic obstructive pulmonary disease patients and sedentary healthy subjects.
|Gouzi F,Préfaut C,Abdellaoui A,Roudier E,de Rigal P,Molinari N,Laoudj-Chenivesse D,Mercier J,Birot O,Hayot M||The European respiratory journal (41:806)||2013|
Chronic delivery of a thrombospondin-1 mimetic decreases skeletal muscle capillarity in mice.
39-9300 was used in western blot to test if chronic exposure to a TSP- mimetic decreases skeletal muscle capillarity and alters angiogenic proteins under basal conditions.
|Audet GN,Fulks D,Stricker JC,Olfert IM||PloS one (8:null)||2013|
Angio-adaptation in unloaded skeletal muscle: new insights into an early and muscle type-specific dynamic process.
39-9300 was used in western blot to investigate how muscle unloading leads to capillary regression
|Roudier E,Gineste C,Wazna A,Dehghan K,Desplanches D,Birot O||The Journal of physiology (588:4579)||2010|
|Human||Not Cited||Localization of the angiogenesis inhibitor thrombospondin in human synovial tissues.||Koch AE,Friedman J,Burrows JC,Haines GK,Bouck NP||Pathobiology : journal of immunopathology, molecular and cellular biology (61:1)||1993|