Immunofluorescence analysis of Thrombospondin 1 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Thrombospondin 1 (A4.1) Mouse Monoclonal Antibody (MA513377) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Rat, Pig, Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13377 targets Thrombospondin in FACS, IF, and WB applications and shows reactivity with Bovine, Chicken, Human, mouse, and Rat samples.
The MA5-13377 immunogen is calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets.
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.
MA5-13377 was used in immunocytochemistry to study the role of a FKBP14 mutation in the pathogenesis of a variant of Ehlers-Danlos syndrome
|Baumann M,Giunta C,Krabichler B,Rüschendorf F,Zoppi N,Colombi M,Bittner RE,Quijano-Roy S,Muntoni F,Cirak S,Schreiber G,Zou Y,Hu Y,Romero NB,Carlier RY,Amberger A,Deutschmann A,Straub V,Rohrbach M,Steinmann B,Rostásy K,Karall D,Bönnemann CG,Zschocke J,Fauth C||American journal of human genetics (90:201)||2012|
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
MA5-13377 was used in immunocytochemistry to investigate the relationship between thrombospondin 2 and Rac1
|Lopes N,Gregg D,Vasudevan S,Hassanain H,Goldschmidt-Clermont P,Kovacic H||Molecular and cellular biology (23:5401)||2003|
Effects of cerivastatin on human arterial smooth muscle cell growth and extracellular matrix expression at varying glucose and low-density lipoprotein levels.
MA5-13377 was used in immunocytochemistry to study the effects of cerivastatin on human arterial smooth muscle cell growth and extracellular matrix at different concentrations of glucose and LDL
|Siegel-Axel DI,Runge H,Seipel L,Riessen R||Journal of cardiovascular pharmacology (41:422)||2003|
Oxidative stress-induced attenuation of thrombospondin-1 expression in primary rat astrocytes.
MA5-13377 was used in western blot to investigate the effect of oxidative stress on thrombospondin 1 expression in astrocytes
|Chen JK,Zhan YJ,Yang CS,Tzeng SF||Journal of cellular biochemistry (112:59)||2011|
Shotgun proteomics implicates extracellular matrix proteins and protease systems in neuronal development induced by astrocyte cholinergic stimulation.
MA5-13377 was used in western blot to identify astrocyte-released proteins involved in neuronal maturation
|Moore NH,Costa LG,Shaffer SA,Goodlett DR,Guizzetti M||Journal of neurochemistry (108:891)||2009|
CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A.
MA5-13377 was used in western blot to investigate the role of the CCAAT box in the induction of human thrombospondin-1 gene by trichostatin A
|Kang JH,Kim MJ,Chang SY,Sim SS,Kim MS,Jo YH||Journal of cellular biochemistry (104:1192)||2008|
Collagen-binding domains of gelatinase A and thrombospondin-derived peptides impede endocytic clearance of active gelatinase A and promote HT1080 fibrosarcoma cell invasion.
MA5-13377 was used in western blot to study the mechanism by which collagen-binding domains of gelatinase A and thrombospondin promote fibrosarcoma cell invasion
|Robinet A,Emonard H,Banyai L,Laronze JY,Patthy L,Hornebeck W,Bellon G||Life sciences (82:376)||2008|
A model of human tumor dormancy: an angiogenic switch from the nonangiogenic phenotype.
MA5-13377 was used in western blot to study the angiogenesis during human tumor progression
|Naumov GN,Bender E,Zurakowski D,Kang SY,Sampson D,Flynn E,Watnick RS,Straume O,Akslen LA,Folkman J,Almog N||Journal of the National Cancer Institute (98:316)||2006|
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
MA5-13377 was used in western blot to study the molecular mechanisms underlying astrocytic-endothelial paracrine signaling
|Cambier S,Gline S,Mu D,Collins R,Araya J,Dolganov G,Einheber S,Boudreau N,Nishimura SL||The American journal of pathology (166:1883)||2005|
Role of thrombospondin 1 in macrophage inflammation in dysferlin myopathy.
MA5-13377 was used in blocking or activating experiment to investigate the influence of TSP-1 on macrophage inflammation in dysferlin myopathy
|De Luna N,Gallardo E,Sonnet C,Chazaud B,Dominguez-Perles R,Suarez-Calvet X,Gherardi RK,Illa I||Journal of neuropathology and experimental neurology (69:643)||2010|
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
MA5-13377 was used in blocking or activating experiment to develop an in vitro model to study factors affecting the migration and proliferation of isolated liver and islet endothelium
|Johansson A,Olerud J,Johansson M,Carlsson PO||Transplant international : official journal of the European Society for Organ Transplantation (22:1182)||2009|
Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.
MA5-13377 was used in blocking or activating experiment to study the regulation of thrombospondin-1 expression by natural and synthetic progestins in human breast cancer cells
|Hyder SM,Liang Y,Wu J,Welbern V||Endocrine-related cancer (16:809)||2009|
Beta1 integrin cytoplasmic variants differentially regulate expression of the antiangiogenic extracellular matrix protein thrombospondin 1.
MA5-13377 was used in blocking/activating experiment to study the effect of beta 1 integrin on the expression of extracellular matrix protein thrombospondin 1
|Goel HL,Moro L,Murphy-Ullrich JE,Hsieh CC,Wu CL,Jiang Z,Languino LR||Cancer research (69:5374)||2009|
Meteorin regulates angiogenesis at the gliovascular interface.
MA5-13377 was used in blocking or activating experiment to investigate the role of meteorin in angiogenesis at the gliovascular interface
|Park JA,Lee HS,Ko KJ,Park SY,Kim JH,Choe G,Kweon HS,Song HS,Ahn JC,Yu YS,Kim KW||Glia (56:247)||2008|
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
MA5-13377 was used in blocking or activating experiment to study the effect of PPARalpha deficiency in inflammatory cells on tumor growth
|Kaipainen A,Kieran MW,Huang S,Butterfield C,Bielenberg D,Mostoslavsky G,Mulligan R,Folkman J,Panigrahy D||PloS one (2:null)||2007|
A new insight into phagocytosis of apoptotic cells: proteolytic enzymes divert the recognition and clearance of polymorphonuclear leukocytes by macrophages.
MA5-13377 was used in blocking or activating experiment to study the in vivo clearance of apoptotic cells by macrophages
|Guzik K,Bzowska M,Smagur J,Krupa O,Sieprawska M,Travis J,Potempa J||Cell death and differentiation (14:171)||2007|
LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity: Role of extracellular proteases plasmin and elastase.
MA5-13377 was used in blocking or activating experiment to study the mechanisms by which LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity
|Gomez-Duran A,Mulero-Navarro S,Chang X,Fernandez-Salguero PM||Journal of cellular biochemistry (97:380)||2006|
NOL7 is a nucleolar candidate tumor suppressor gene in cervical cancer that modulates the angiogenic phenotype.
MA5-13377 was used in blocking or activating experiment to study the role of NOL7 in cervical cancer
|Hasina R,Pontier AL,Fekete MJ,Martin LE,Qi XM,Brigaudeau C,Pramanik R,Cline EI,Coignet LJ,Lingen MW||Oncogene (25:588)||2006|
Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.
MA5-13377 was used in blocking or activating experiment to investigate the mechanism of endocytic clearance mediated by low density lipoprotein receptor-related protein
|Emonard H,Bellon G,Troeberg L,Berton A,Robinet A,Henriet P,Marbaix E,Kirkegaard K,Patthy L,Eeckhout Y,Nagase H,Hornebeck W,Courtoy PJ||The Journal of biological chemistry (279:54944)||2004|
Thrombospondin 1, a mediator of the antiangiogenic effects of low-dose metronomic chemotherapy.
MA5-13377 was used in blocking or activating experiment to study the contribution of thrombospondin-1 to the antiangiogenic effects of low-dose metronomic chemotherapy
|Bocci G,Francia G,Man S,Lawler J,Kerbel RS||Proceedings of the National Academy of Sciences of the United States of America (100:12917)||2003|
Thrombospondin-1 promotes fibroblast-mediated collagen gel contraction caused by activation of latent transforming growth factor beta-1.
MA5-13377 was used in blocking or activating experiment to study the role of thrombospondin-1 in fibroblast-mediated collagen gel contraction
|Sakai K,Sumi Y,Muramatsu H,Hata K,Muramatsu T,Ueda M||Journal of dermatological science (31:99)||2003|
Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.
MA5-13377 was used in blocking or activating experiment to investigate the adhesion of erythrocytes during deep vein thrombosis
|Goel MS,Diamond SL||Blood (100:3797)||2002|
Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts.
MA5-13377 was used in blocking or activating experiment to study the role of thrombospondin-1 in the supression of metastasis by the primary tumor in a human melanoma xenograft model
|Rofstad EK,Graff BA||The Journal of investigative dermatology (117:1042)||2001|
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
MA5-13377 was used in blocking or activating experiment to study the mechanism by which thrombospondin mediates the adhesion of sickle erythrocytes to endothelium
|Gupta K,Gupta P,Solovey A,Hebbel RP||Biochimica et biophysica acta (1453:63)||1999|
Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degeneration.
MA5-13377 was used in immunohistochemistry to study thrombospondin-1-dependent microvascular degeneration induced by transarachidonic acids during nitrative stress.
|Kermorvant-Duchemin E,Sennlaub F,Sirinyan M,Brault S,Andelfinger G,Kooli A,Germain S,Ong H,d'Orleans-Juste P,Gobeil F,Zhu T,Boisvert C,Hardy P,Jain K,Falck JR,Balazy M,Chemtob S||Nature medicine (11:1339)||2005|
Antiangiogenic treatment with thrombospondin-1 enhances primary tumor radiation response and prevents growth of dormant pulmonary micrometastases after curative radiation therapy in human melanoma xenografts.
MA5-13377 was used in immunohistochemistry to study the effect of thrombospondin-1 treatment on the response to radiation therapy in human melanoma xenografts
|Rofstad EK,Henriksen K,Galappathi K,Mathiesen B||Cancer research (63:4055)||2003|
Inhibiting cell proliferation during formation of the glial scar: effects on axon regeneration in the CNS.
MA5-13377 was used in immunohistochemistry to study the effect of inhibiting cell proliferation on glial scar formation and CNS axon regeneration
|Rhodes KE,Moon LD,Fawcett JW||Neuroscience (120:41)||2003|