|Tested species reactivity||Bovine, Dog, Horse, Human, Sheep, Pig, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13390 targets Thrombospondin in FACS, IF, IM, IP, and WB applications and shows reactivity with Bovine, Canine, Equine, Human, Ovine, Porcine, and Rat samples.
The MA5-13390 immunogen is ca2+-replete, native purified human TSP from supernatant of thrombin-activated platelets.
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Adipose-derived stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the formation of microvascular networks.
MA5-13390 was used in western blot to study the effect of advancing age on the capacity of the stromal vascular fraction to stimulate neovascularization.
|Aird AL,Nevitt CD,Christian K,Williams SK,Hoying JB,LeBlanc AJ||Experimental gerontology (63:18)||2015|
Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.
MA5-13390 was used in western blot to study the roles of LRP-1 and thrombospondin -1 in regulating the motility and adhesion/de-adhesion of T-cells
|Talme T,Bergdahl E,Sundqvist KG||Immunology (142:176)||2014|
A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.
MA5-13390 was used in blocking or activating experiment and western blot to study the mechanism underlying cytokine-mediated modulation of the motility, adhesion and activation of T-cells
|Bergström SE,Bergdahl E,Sundqvist KG||Immunology (140:441)||2013|
Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.
MA5-13390 was used in blocking or activating experiment to study the modulation of thrombospondin-1 levels in astrocytes by morphine and its implications for neurite outgrowth and synapse formation
|Ikeda H,Miyatake M,Koshikawa N,Ochiai K,Yamada K,Kiss A,Donlin MJ,Panneton WM,Churchill JD,Green M,Siddiqui AM,Leinweber AL,Crews NR,Ezerskiy LA,Rendell VR,Belcheva MM,Coscia CJ||The Journal of biological chemistry (285:38415)||2010|
Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain.
MA5-13390 was used in blocking or activating experiment to investigate the role of the heparin-binding domain of the phosphatidylserine-positive erythrocytes in its binding to immobilized and soluble thrombosphodin-1
|Gayen Betal S,Setty BN||Translational research : the journal of laboratory and clinical medicine (152:165)||2008|
Thrombospondin-1 and indoleamine 2,3-dioxygenase are major targets of extracellular ATP in human dendritic cells.
MA5-13390 was used in blocking or activating experiment to investigate the effect of ATP on the expression of the indoleamine 2,3-dioxygenase and kynurenine production in human dendritic cells
|Marteau F,Gonzalez NS,Communi D,Goldman M,Boeynaems JM,Communi D||Blood (106:3860)||2005|
Novel CD47-dependent intercellular adhesion modulates cell migration.
MA5-13390 was used in blocking or activating experiment to study cell migration modulated by CD47-dependent intercellular adhesion
|Rebres RA,Kajihara K,Brown EJ||Journal of cellular physiology (205:182)||2005|
Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.
MA5-13390 was used in blocking or activating experiment to investigate the mechanism of endocytic clearance mediated by low density lipoprotein receptor-related protein
|Emonard H,Bellon G,Troeberg L,Berton A,Robinet A,Henriet P,Marbaix E,Kirkegaard K,Patthy L,Eeckhout Y,Nagase H,Hornebeck W,Courtoy PJ||The Journal of biological chemistry (279:54944)||2004|
CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum.
MA5-13390 was used in blocking or activating experiment to study the role of CD36 in the non-opsonic phagocytosis of erythrocytes infected with Plasmodium falciparum gametocytes
|Smith TG,Serghides L,Patel SN,Febbraio M,Silverstein RL,Kain KC||Infection and immunity (71:393)||2003|
Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.
MA5-13390 was used in blocking or activating experiment to investigate the adhesion of erythrocytes during deep vein thrombosis
|Goel MS,Diamond SL||Blood (100:3797)||2002|
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
MA5-13390 was used in blocking or activating experiment to study the mechanism by which thrombospondin mediates the adhesion of sickle erythrocytes to endothelium
|Gupta K,Gupta P,Solovey A,Hebbel RP||Biochimica et biophysica acta (1453:63)||1999|
Bystander senescence in human peritoneal mesothelium and fibroblasts is related to thrombospondin-1-dependent activation of transforming growth factor-ß1.
MA5-13390 was used in ELISA to study the role of thrombospondin-1 and TGF-beta1 in bystander senescence in human peritoneal mesothelium and fibroblasts
|Miku¿a-Pietrasik J,Sosi¿ska P,Janus J,Rubi¿ B,Brewi¿ska-Olchowik M,Piwocka K,Ksi¿¿ek K||The international journal of biochemistry and cell biology (45:2087)||2013|
Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations.
MA5-13390 was used in ELISA to probe the structure of thrombospondin-1 and thrombospondin-2 signature domains at low calcium concentration using conformation-sensitive monoclonal antibodies
|Annis DS,Gunderson KA,Mosher DF||The Journal of biological chemistry (282:27067)||2007|
|Not Applicable||Not Cited||
Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.
MA5-13390 was used in immunocytochemistry to study the role of the calreticulin-thrombospondin-1 interaction in autocrine regulation of T cell motility
|Li SS,Forslöw A,Sundqvist KG||Journal of immunology (Baltimore, Md. : 1950) (174:654)||2005|
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
MA5-13390 was used in immunocytochemistry to investigate the relationship between thrombospondin 2 and Rac1
|Lopes N,Gregg D,Vasudevan S,Hassanain H,Goldschmidt-Clermont P,Kovacic H||Molecular and cellular biology (23:5401)||2003|
Thrombospondin 1 is an autocrine negative regulator of human dendritic cell activation.
MA5-13390 was used in flow cytometry to investigate the role of thrombospondin 1 in human dendritic cell activation
|Doyen V,Rubio M,Braun D,Nakajima T,Abe J,Saito H,Delespesse G,Sarfati M||The Journal of experimental medicine (198:1277)||2003|