|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His-tagged recombinant human thymidine kinase.|
|Storage buffer||PBS, pH 7.0|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21542 detects Thymidine Kinase in Human samples.
MA1-21542 has been successfully used in ELISA and Western Blot procedures.
MA1-21542 immunogen corresponds to His-tagged recombinant human thymidine kinase.
Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. High levels of these enzymes are present in proliferating cells, and low levels are found in resting cells, due to multiple regulatory mechanisms that ensure exclusive expression of these enzymes in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.