Immunofluorescent analysis of Thymidylate synthetase showing staining in the cytoplasm of A375 cells. A375 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a Thymidylate synthetase polyclonal antibody (Product # PA5-27867) diluted at 1:1000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 35 and 313 of Human Thymidylate synthetase|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27867 targets Thymidylate synthetase in IHC (P) and WB applications and shows reactivity with Human, rat, and mouse samples.
The PA5-27867 immunogen is recombinant fragment corresponding to a region within amino acids 35 and 313 of Human Thymidylate synthetase.
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
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