Immunofluorescent analysis of Thyroid Hormone Receptor alpha-2 (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thyroid Hormone Receptor alpha-2 polyclonal antibody (Product # PA1-216) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-216 detects the thyroid hormone receptor alpha 2 from human and mouse samples.
PA1-216 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects bands at ~58 kDa representing Tra2 in mouse tissue. This antibody also detects a ~43 kDa protein which is believed to be non-specific.
The PA1-216 immunizing peptide corresponds to amino acid residues 455-469 from rat thyroid hormone receptor alpha 2.
Thyroid hormone receptor alpha 2 protein mediates the function of thyroid hormone. There are three forms of the thyroid hormone receptors known as alpha-1, alpha-2 and beta. Studies have suggested that these three forms of the thyroid hormone receptor are abundant in most tissues; however, none of these three thyroid hormone receptors were abundant in the liver, which is the major thyroid hormone-responsive organ. Therefore another type of thyroid hormone receptor is believed to be present in liver.
The identification of the several types of thyroid hormone receptor may explain the normal variation in thyroid hormone responsiveness of various organs and the selective tissue abnormalities found in the thyroid hormone resistance syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aging impairs myocardial fatty acid and ketone oxidation and modifies cardiac functional and metabolic responses to insulin in mice.
PA1-216 was used in western blot to investigate the effect of aging on myocardial fatty acid, ketone oxidation, and insulin sensitivity in mice
|Hyyti OM,Ledee D,Ning XH,Ge M,Portman MA||American journal of physiology. Heart and circulatory physiology (299:H868)||2010|
Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.
PA1-216 was used in western blot to investigate the role of thyroid hormone on cardiac remodeling and myocardial performance in rat
|Pantos C,Mourouzis I,Markakis K,Dimopoulos A,Xinaris C,Kokkinos AD,Panagiotou M,Cokkinos DV||European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (32:333)||2007|
Thyroid hormone receptor isoforms localize to cardiac mitochondrial matrix with potential for binding to receptor elements on mtDNA.
PA1-216 was used in western blot to study teh localization of thyroid hormone receptor isoforms in cardiac mitochondrial matrix
|Morrish F,Buroker NE,Ge M,Ning XH,Lopez-Guisa J,Hockenbery D,Portman MA||Mitochondrion (6:143)||2006|