Immunohistochemical analysis of Thyroid Hormone Receptor alpha1/alpha2 using anti-Thyroid Hormone Receptor alpha1/alpha2 Polyclonal Antibody (PA5-32615) in Thyroid Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human TR-alpha 1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:150|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is thyroid tissue.
Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TRalpha and TRBeta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 and quote;-triiodo-L-thyronine (T-3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human parvovirus B19 infection leads to downregulation of thyroid, estrogen, and retinoid hormone receptor expression.
PA5-32615 was used in immunocytochemistry to study the downregulation of thyroid, estrogen and retinoid hormone receptors in erythroid cells by human parvovirus B19 infection
|Ignatovich IV,Hobbs JA||Virology (446:173)||2013|