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Immunohistochemistry analysis of Thyroid Hormone Receptor beta-1 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thyroid Hormone Receptor beta-1 Mouse Monoclonal Antibody (MA1-216) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human TR beta -1 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-216 detects thyroid hormone receptor (TR) beta-1 in human, mouse, rat, and transformed bacterial cells. This antibody is specific for the TR beta-1 isoform.
MA1-216 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects a 52 and a 55 kDa protein representing recombinant TR expressed in E. coli. The two different sizes represent two different translational starting points. Immunocytochemical staining of TR beta-1 in intact GH3 cells with MA1-216 results in nuclear staining. When levels of expression of the transfected receptor are high, cytoplasmic staining is seen along with nuclear staining. Immunoprecipitation and gel super shift experiments show that MA1-216 reacts with the unliganded, liganded, and DNA binding forms of the receptor.
The MA1-216 immunogen is purified human TR beta-1 overexpressed in E. coli. This antibody recognizes an epitope in the A/B domain of human TR beta-1, amino acid residues 1-101.
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.
The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.
MA1-216 was used in western blot to investigate thyroid hormone receptor in adipogenesis
|Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA||The Journal of biological chemistry (290:7402)||2015|
Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats.
MA1-216 was used in western blot to study the effect of permanent cerebral ischemia on the expression of thyroid hormone receptors in a male rat model
|Lourbopoulos A,Mourouzis I,Karapanayiotides T,Nousiopoulou E,Chatzigeorgiou S,Mavridis T,Kokkinakis I,Touloumi O,Irinopoulou T,Chouliaras K,Pantos C,Karacostas D,Grigoriadis N||Journal of molecular neuroscience : MN (54:78)||2014|
Thyroid hormone signalling is altered in response to physical training in patients with end-stage heart failure and mechanical assist devices: potential physiological consequences?
MA1-216 was used in western blot to study the enhanced thyroid signalling observed in end-stage heart failure patients with ventricular assist devices following exercise training
|Adamopoulos S,Gouziouta A,Mantzouratou P,Laoutaris ID,Dritsas A,Cokkinos DV,Mourouzis I,Sfyrakis P,Iervasi G,Pantos C||Interactive cardiovascular and thoracic surgery (17:664)||2013|
Inhibition of thyroid hormone receptor α1 impairs post-ischemic cardiac performance after myocardial infarction in mice.
MA1-216 was used in western blot to study the deleterious effect of pharmacological inhibition of thyroid hormone receptor alpha1 on post-ischemic cardiac function in a murine model of myocardial infarction
|Mourouzis I,Kostakou E,Galanopoulos G,Mantzouratou P,Pantos C||Molecular and cellular biochemistry (379:97)||2013|
Cell-type-dependent thyroid hormone effects on glioma tumor cell lines.
MA1-216 was used in western blot to study the effect of thyroid hormone on glioma cells
|Liappas A,Alexandros L,Mourouzis I,Iordanis M,Zisakis A,Athanasios Z,Economou K,Konstantinos E,Lea RW,Robert-William L,Pantos C,Constantinos P||Journal of thyroid research (2011:null)||2012|
Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction.
MA1-216 was used in western blot to investigate the effect of thyroid hormone on remodelling the diabetic myocardium
|Kalofoutis C,Mourouzis I,Galanopoulos G,Dimopoulos A,Perimenis P,Spanou D,Cokkinos DV,Singh J,Pantos C||Molecular and cellular biochemistry (345:161)||2010|
Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy.
MA1-216 was used in western blot to investigate the possible mechanism of the pathogenesis of DCM caused by hyperthyroidism using a konck-in mouse model
|Wang YY,Morimoto S,Du CK,Lu QW,Zhan DY,Tsutsumi T,Ide T,Miwa Y,Takahashi-Yanaga F,Sasaguri T||Cardiovascular research (87:636)||2010|
TNF-alpha administration in neonatal cardiomyocytes is associated with differential expression of thyroid hormone receptors: a response prevented by T3.
MA1-216 was used in western blot to investigate the effect of tumor necrosis factor alpha administration on thyroid hormone receptors in neonatal cardiomyocytes
|Pantos C,Xinaris C,Mourouzis I,Kokkinos AD,Cokkinos DV||Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et me¿tabolisme (40:731)||2008|
Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.
MA1-216 was used in western blot to investigate the role of thyroid hormone on cardiac remodeling and myocardial performance in rat
|Pantos C,Mourouzis I,Markakis K,Dimopoulos A,Xinaris C,Kokkinos AD,Panagiotou M,Cokkinos DV||European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (32:333)||2007|
Time-dependent changes in the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction: possible implications in cardiac remodelling.
MA1-216 was used in western blot to investigate the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction
|Pantos C,Mourouzis I,Xinaris C,Kokkinos AD,Markakis K,Dimopoulos A,Panagiotou M,Saranteas T,Kostopanagiotou G,Cokkinos DV||European journal of endocrinology / European Federation of Endocrine Societies (156:415)||2007|
Thyroid hormone receptors alpha1 and beta1 are downregulated in the post-infarcted rat heart: consequences on the response to ischaemia-reperfusion.
MA1-216 was used in western blot to investigate the function of thyroid hormone signaling in the response of heart to ischaemia-reperfusion after myocardial infarction
|Pantos C,Mourouzis I,Saranteas T,Paizis I,Xinaris C,Malliopoulou V,Cokkinos DV||Basic research in cardiology (100:422)||2005|
Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity.
MA1-216 was used in western blot to demonstrate the cell cycle-dependent expression of thyroid hormone receptor-beta.
|Maruvada P,Dmitrieva NI,East-Palmer J,Yen PM||Molecular biology of the cell (15:1895)||2004|
Increased expression of thyroid hormone receptor isoforms in end-stage human congestive heart failure.
MA1-216 was used in western blot to investigate the expression status of thyroid hormone receptor isoforms in end-stage human myocardial failure .
|d'Amati G,di Gioia CR,Mentuccia D,Pistilli D,Proietti-Pannunzi L,Miraldi F,Gallo P,Celi FS||The Journal of clinical endocrinology and metabolism (86:2080)||2001|
Overexpression of thyroid hormone receptor beta1 is associated with thyrotropin receptor gene expression and proliferation in a human thyroid carcinoma cell line.
MA1-216 was used in western blot to investigate the association of thyroid hormone receptor beta1 and thyrotropin receptor
|Chen ST,Shieh HY,Lin JD,Chang KS,Lin KH||The Journal of endocrinology (165:379)||2000|
Thyroid hormone affects Schwann cell and oligodendrocyte gene expression at the glial transition zone of the VIIIth nerve prior to cochlea function.
MA1-216 was used in western blot to examine the effects of thyroid hormone on the expression of oligodendrocyte gene at the glial transition zone of the VIIIth nerve
|Knipper M,Bandtlow C,Gestwa L,Köpschall I,Rohbock K,Wiechers B,Zenner HP,Zimmermann U||Development (Cambridge, England) (125:3709)||1998|
Genome-wide binding patterns of thyroid hormone receptor beta.
MA1-216 was used in ChIP assay and EMSA to perform a genome-wide analysis of thyroid hormone receptor-beta binding using BioChIP in HepG2 cells
|Ayers S,Switnicki MP,Angajala A,Lammel J,Arumanayagam AS,Webb P||PloS one (9:null)||2014|
Nuclear corepressors mediate the repression of phospholipase A2 group IIa gene transcription by thyroid hormone.
MA1-216 was used in ChIP assay to study the mechanism by which thyroid hormone T3 inhibits the expression of secretory phospholipase A2 group IIa
|Sharma P,Thakran S,Deng X,Elam MB,Park EA||The Journal of biological chemistry (288:16321)||2013|
Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by CCAAT/enhancer-binding protein beta (C/EBPbeta).
MA1-216 was used in ChIP assay to investigate the importance of CCAAT/enhancer-binding protein beta in the regulation of pyruvate dehydrogenase kinase 4 expression
|Attia RR,Sharma P,Janssen RC,Friedman JE,Deng X,Lee JS,Elam MB,Cook GA,Park EA||The Journal of biological chemistry (286:23799)||2011|
p62, A TFIIH subunit, directly interacts with thyroid hormone receptor and enhances T3-mediated transcription.
MA1-216 was used in chromatin immunoprecipitation to study the interaction between the TFIIH subunit p62 and thyroid hormone receptor and its effect on gene transcription.
|Liu Y,Ando S,Xia X,Yao R,Kim M,Fondell J,Yen PM||Molecular endocrinology (Baltimore, Md.) (19:879)||2005|
Triiodothyronine accelerates differentiation of rat liver progenitor cells into hepatocytes.
MA1-216 was used in immunohistochemistry to investigate the effect of triiodothyronine on rat liver hepatocyte development
|László V,Dezso K,Baghy K,Papp V,Kovalszky I,Sáfrány G,Thorgeirsson SS,Nagy P,Paku S||Histochemistry and cell biology (130:1005)||2008|
Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow.
MA1-216 was used in immunohistochemistry to investigate the association between thyroid status and thyroid hormone receptor expressing mast cells
|Siebler T,Robson H,Bromley M,Stevens DA,Shalet SM,Williams GR||Bone (30:259)||2002|
Variable transcriptional activity and ligand binding of mutant beta 1 3,5,3'-triiodothyronine receptors from four families with generalized resistance to thyroid hormone.
MA1-216 was used in immunocytochemistry to characterize TR beta 1 mutants
|Meier CA,Dickstein BM,Ashizawa K,McClaskey JH,Muchmore P,Ransom SC,Menke JB,Hao EH,Usala SJ,Bercu BB||Molecular endocrinology (Baltimore, Md.) (6:248)||1992|
c-erbA-2; c-erbA-beta; nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; THRB; thyroid hormone nuclear receptor beta variant 1; thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR beta; TR Beta 1; TRbeta2Delta
C-ERBA-2; C-ERBA-BETA; c-erbAbeta; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3R[b]; T3Rbeta; T3rec; THR1; THRB; THRB1; THRB2; TRbeta