|Tested species reactivity||Chicken, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified, full length, native, chicken titin protein from chicken breast muscle.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22379 detects titin in chicken and rat samples.
MA1-22379 has been successfully used in immunofluorescence, immunohistochemistry (frozen), and Western blot procedures.
The MA1-22379 immunogen is purified, full length, native, chicken titin protein from chicken breast muscle.
Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles.
Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.