|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length protein (Human).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA1-21150 detects Topoisomerase II beta in Human samples.
PA1-21150 has been successfully used in Immunohistochemistry (Paraffin) procedures.
PA1-21150 immunogen corresponds to Recombinant full length protein (Human)..
Topoisomerase II beta plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II beta is also implicated in drug resistance of tumour cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria.
PA1-21150 was used in immunoprecipitation and western blot to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion
|Zhang H,Zhang YW,Yasukawa T,Dalla Rosa I,Khiati S,Pommier Y||Nucleic acids research (42:7259)||2014|