Immunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
|Tested species reactivity||Human, Non-human primate, Rat|
|Published species reactivity||Rat, Non-human primate, Hamster, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues L(1) P S A S K P N N T S S E N N P P(17) C of rat TGN38.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/20 - 1/50|
|Immunofluorescence (IF)||1:50 - 1:200|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-063 detects trans-Golgi network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
MA3-063 has been successfully used in Western blot, immunofluorescence, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. Immunofluorescence staining of TGN38 in NRK cells with MA3-063 results in staining predominantly in the TGN.
In immunofluorescence, it has been shown that methanol works best as a fixative.
The MA3-063 immunogen is a synthetic peptide corresponding to residues L(1) P S A S K P N N T S S E N N P P(17) C of rat TGN38.
The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways.
TGN38 and TGN41, an isoform of the former, are integral membrane proteins which are predominantly localized to the TGN in NRK cells but which can also be observed at the cell surface. Complexes which include TGN38, low molecular weight G proteins and p62 are required for the formation of TGN-derived vesicles. This requirement has lead people to believe that TGN38 is important in the trafficking of proteins from the TGN to the cell surface. Brefeldin A causes the contents of the Golgi stack to be redistributed into the endoplasmic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38 following brefeldin A treatment a useful tool for the discrimination of the TGN from the Golgi stack.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
mRNA redistribution during permanent focal cerebral ischemia.
MA3-063 was used in immunohistochemistry to study mRNA granule formation following experimental permanent focal cerebral ischemia
|Lewis MK,Jamison JT,Dunbar JC,DeGracia DJ||Translational stroke research (4:604)||2013|
Activated somatostatin type 2 receptors traffic in vivo in central neurons from dendrites to the trans Golgi before recycling.
MA3-063 was used in immunohistochemistry to investigate the trafficking and recycling of activated somatostatin type 2 receptors in vivo in central neurons
|Csaba Z,Lelouvier B,Viollet C,El Ghouzzi V,Toyama K,Videau C,Bernard V,Dournaud P||Traffic (Copenhagen, Denmark) (8:820)||2007|
A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles.
MA3-063 was used in immunohistochemistry to investigate the function of cytoplasmic autoantigen GW182 within cytoplasmic speckles
|Eystathioy T,Chan EK,Tenenbaum SA,Keene JD,Griffith K,Fritzler MJ||Molecular biology of the cell (13:1338)||2002|
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
MA3-063 was used in immunohistochemistry to study the mechanism in CCR5 cell surface expression.
|Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM||The Journal of biological chemistry (276:40133)||2001|
Sphingomyelin-enriched microdomains at the Golgi complex.
MA3-063 was used in immunohistochemistry to investigate the roles of the sphingomyelin-enriched microdomains in the Golgi complex.
|Gkantiragas I,Brügger B,Stüven E,Kaloyanova D,Li XY,Löhr K,Lottspeich F,Wieland FT,Helms JB||Molecular biology of the cell (12:1819)||2001|
Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro.
MA3-063 was used in immunocytochemistry to study the mechanism of the receptor-mediated transcytosis of Leptin
|Cammisotto PG,Bendayan M,Sané A,Dominguez M,Garofalo C,Levy E||International journal of cell biology (2010:null)||2010|
Dynamics of somatostatin type 2A receptor cargoes in living hippocampal neurons.
MA3-063 was used in immunocytochemistry to investigate the intracellular trafficking of somatostatin type 2A receptor cargoes in living hippocampal neurons.
|Lelouvier B,Tamagno G,Kaindl AM,Roland A,Lelievre V,Le Verche V,Loudes C,Gressens P,Faivre-Baumann A,Lenkei Z,Dournaud P||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:4336)||2008|
Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.
MA3-063 was used in immunocytochemistry to study the gap junction formation and degradation in human prostate cancer cells.
|Mitra S,Annamalai L,Chakraborty S,Johnson K,Song XH,Batra SK,Mehta PP||Molecular biology of the cell (17:5400)||2006|
The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly.
MA3-063 was used in immunocytochemistry to study the association between IFT20 and the Golgi complex during cilia assembly
|Follit JA,Tuft RA,Fogarty KE,Pazour GJ||Molecular biology of the cell (17:3781)||2006|
Protein kinase D intracellular localization and activity control kinase D-interacting substrate of 220-kDa traffic through a postsynaptic density-95/discs large/zonula occludens-1-binding motif.
MA3-063 was used in immunocytochemistry to investigate the molecular mechanism for the protein kinase D controls on protein traffic .
|Sánchez-Ruiloba L,Cabrera-Poch N,Rodríguez-Martínez M,López-Menéndez C,Jean-Mairet RM,Higuero AM,Iglesias T||The Journal of biological chemistry (281:18888)||2006|
Cholesterol-regulated translocation of NPC1L1 to the cell surface facilitates free cholesterol uptake.
MA3-063 was used in immunocytochemistry to investigate the role of NPC1L1 in regulating cellular cholesterol uptake.
|Yu L,Bharadwaj S,Brown JM,Ma Y,Du W,Davis MA,Michaely P,Liu P,Willingham MC,Rudel LL||The Journal of biological chemistry (281:6616)||2006|
Plasma membrane residence of hyaluronan synthase is coupled to its enzymatic activity.
MA3-063 was used in immunocytochemistry to investigate the intracellular transport of hyaluronan synthase.
|Rilla K,Siiskonen H,Spicer AP,Hyttinen JM,Tammi MI,Tammi RH||The Journal of biological chemistry (280:31890)||2005|
Regulation of microtubule-dependent recycling at the trans-Golgi network by Rab6A and Rab6A'.
MA3-063 was used in immunocytochemistry to study the effect of Rab6A and Rab6A' on microtubule-dependent recycling at the trans-Golgi network
|Young J,Stauber T,del Nery E,Vernos I,Pepperkok R,Nilsson T||Molecular biology of the cell (16:162)||2005|
Transactivation of Trk neurotrophin receptors by G-protein-coupled receptor ligands occurs on intracellular membranes.
MA3-063 was used in immunocytochemistry to study the mechanism for Trk neurotrophin receptor transactivation by G-protein-coupled receptor ligands .
|Rajagopal R,Chen ZY,Lee FS,Chao MV||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:6650)||2004|
Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells.
MA3-063 was used in immunocytochemistry to investigate the CRHSP-28 function regulation in acinar cells
|Thomas DD,Weng N,Groblewski GE||American journal of physiology. Gastrointestinal and liver physiology (287:G253)||2004|
Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.
MA3-063 was used in immunocytochemistry to investigate the role of syntaxin 6 in endosomal function in pancreatic beta-cells
|Kuliawat R,Kalinina E,Bock J,Fricker L,McGraw TE,Kim SR,Zhong J,Scheller R,Arvan P||Molecular biology of the cell (15:1690)||2004|
Dispersal of Golgi matrix proteins during mitotic Golgi disassembly.
MA3-063 was used in immunocytochemistry to study the localization of golgins and GRASPs in mitotic Golgi clusters.
|Puri S,Telfer H,Velliste M,Murphy RF,Linstedt AD||Journal of cell science (117:451)||2004|
Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.
MA3-063 was used in immunocytochemistry to investigate the mechanism of endocytosis of adherens junction proteins and tight junction proteins in T84 epithelial cells
|Ivanov AI,Nusrat A,Parkos CA||Molecular biology of the cell (15:176)||2004|
Gap junction-microtubule associations in rat alveolar epithelial cells.
MA3-063 was used in immunocytochemistry to investigate the association of gap junction with microtubules in rat alveolar epithelial cells
|Guo Y,Martinez-Williams C,Rannels DE||American journal of physiology. Lung cellular and molecular physiology (285:L1213)||2003|
Inhibition of a Golgi complex lysophospholipid acyltransferase induces membrane tubule formation and retrograde trafficking.
MA3-063 was used in immunocytochemistry to investigate the role of golgi complex lysophospholipid acyltransferase (LPAT) on membrane tubule formation and retrograde trafficking
|Drecktrah D,Chambers K,Racoosin EL,Cluett EB,Gucwa A,Jackson B,Brown WJ||Molecular biology of the cell (14:3459)||2003|
Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the membrane trafficking inhibitor brefeldin A.
MA3-063 was used in immunocytochemistry to investigate the dynamics of the Golgi apparatus during murine oocyte in vitro maturation
|Moreno RD,Schatten G,Ramalho-Santos J||Biology of reproduction (66:1259)||2002|
Proprotein convertase PC5 regulation by PDGF-BB involves PI3-kinase/p70(s6)-kinase activation in vascular smooth muscle cells.
MA3-063 was used in immunocytochemistry to study how PC5 is regulated in vascular smooth muscle cells in vitro and in vivo
|Stawowy P,Blaschke F,Kilimnik A,Goetze S,Kallisch H,Chrétien M,Marcinkiewicz M,Fleck E,Graf K||Hypertension (Dallas, Tex. : 1979) (39:399)||2002|
Acid prohormone sequence determines size, shape, and docking of secretory vesicles in atrial myocytes.
MA3-063 was used in immunocytochemistry to investigate the mechanism for the production of vesicles in the trans-Golgi network and for the docking at the plasma membrane
|Baertschi AJ,Monnier D,Schmidt U,Levitan ES,Fakan S,Roatti A||Circulation research (89:E23)||2001|
Glucocorticoid induction of the glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues.
MA3-063 was used in immunocytochemistry to study the effect of glucocorticoid on glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues.
|Clark AF,Steely HT,Dickerson JE,English-Wright S,Stropki K,McCartney MD,Jacobson N,Shepard AR,Clark JI,Matsushima H,Peskind ER,Leverenz JB,Wilkinson CW,Swiderski RE,Fingert JH,Sheffield VC,Stone EM||Investigative ophthalmology and visual science (42:1769)||2001|
Protein tyrosine phosphatase phi regulates paxillin tyrosine phosphorylation and mediates colony-stimulating factor 1-induced morphological changes in macrophages.
MA3-063 was used in immunocytochemistry to study the effect of protein tyrosine phosphatase phi on paxillin tyrosine phosphorylation and its role in the effect of colony-stimulating factor 1 on macrophage morphology.
|Pixley FJ,Lee PS,Condeelis JS,Stanley ER||Molecular and cellular biology (21:1795)||2001|
Inhibition of secretion by 1,3-Cyclohexanebis(methylamine), a dibasic compound that interferes with coatomer function.
MA3-063 was used in immunocytochemistry to study the effects of 1, 3-cyclohexanebis (methylamine) (CBM) on secretion
|Hu T,Kao CY,Hudson RT,Chen A,Draper RK||Molecular biology of the cell (10:921)||1999|
Endoplasmic reticulum and trans-Golgi network generate distinct populations of Alzheimer beta-amyloid peptides.
MA3-063 was used in immunocytochemistry to investigate the generation of Alzheimer beta-amyloid peptides in different subcellular compartments
|Greenfield JP,Tsai J,Gouras GK,Hai B,Thinakaran G,Checler F,Sisodia SS,Greengard P,Xu H||Proceedings of the National Academy of Sciences of the United States of America (96:742)||1999|
Vacuolar ATPase inactivation blocks recycling to the trans-Golgi network from the plasma membrane.
MA3-063 was used in immunocytochemistry to study the effect of vacuolar ATPase inactivation on Golgi-plasma membrane recycling
|Reaves B,Banting G||FEBS letters (345:61)||1994|
|Non-human primate||Not Cited||
A membrane-proximal region in the C-terminal tail of NHE7 is required for its distribution in the trans-Golgi network, distinct from NHE6 localization at endosomes.
MA3-063 was used in immunocytochemistry to identify the intramolecular region involved in the specific localization of NHE6 and NHE7
|Fukura N,Ohgaki R,Matsushita M,Nakamura N,Mitsui K,Kanazawa H||The Journal of membrane biology (234:149)||2010|
Lipids and glycosphingolipids in caveolae and surrounding plasma membrane of primary rat adipocytes.
MA3-063 was used in western blot to analyze lipids and glycosphingolipids in and around caveolae of primary rat adipocytes
|Ortegren U,Karlsson M,Blazic N,Blomqvist M,Nystrom FH,Gustavsson J,Fredman P,Strålfors P||European journal of biochemistry (271:2028)||2004|
CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin.
MA3-063 was used in western blot to examine the subcellular localization of the alternatively spliced product CASP
|Gillingham AK,Pfeifer AC,Munro S||Molecular biology of the cell (13:3761)||2002|
Intracellular assembly of very low density lipoproteins containing apolipoprotein B100 in rat hepatoma McA-RH7777 cells.
MA3-063 was used in western blot to investigate the VLDL assembly in rat hepatoma McA-RH7777 cells.
|Tran K,Thorne-Tjomsland G,DeLong CJ,Cui Z,Shan J,Burton L,Jamieson JC,Yao Z||The Journal of biological chemistry (277:31187)||2002|
Presenilin 1 is required for maturation and cell surface accumulation of nicastrin.
MA3-063 was used in western blot to investigate the role of presenilin 1 during the maturation and cell surface accumulation of nicastrin.
|Leem JY,Vijayan S,Han P,Cai D,Machura M,Lopes KO,Veselits ML,Xu H,Thinakaran G||The Journal of biological chemistry (277:19236)||2002|