|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues V(252) V P E Q P S W K D H S K P I S(267) of human TGN46.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:250 to 1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1069 detects TGN46 in human samples.
PA1-1069 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a
~51 kDa protein representing TGN46 in HEK293 and HeLa cell lysates.
The PA1-1069 immunogen is a synthetic peptide corresponding to residues V(252) V P E Q P S W K D H S K P I S(267) of human TGN46. This peptide (Cat. # PEP-291) is available for use in neutralization and control experiments.
The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways.
TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are exist, TGN46, TGN48 and TGN51. Isoform TGN46 is the more widely expressed isoform. TGN48 is expressed in embryonic kidney and promyelocytic cells. Isoform TGN51 is abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.