|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Transactivation/retinoblastoma protein binding domain (a.a. 332-390) of E2F-1 expressed in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23202 detects E2F1 in Human samples.
MA1-23202 has been successfully used in Immunoprecipitation and Western Blot procedures. A suggested positive control is E2F-1 transfected Soas2 cells.
MA1-23202 immunogen corresponds to Transactivation/retinoblastoma protein binding domain (a.a. 332-390) of E2F-1 expressed in E. coli.
Store at 4°C short term. For extended storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
E2F-1-17E3 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.