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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein.|
|Storage buffer||PBS, pH 7.0-7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||4-8 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25333 detects E2F3 from human samples.
MA1-25333 has been successfully used in immunoprecipitation, gel shift and Western blot procedures.
The MA1-25333 immunogen is recombinant human protein.
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DKFZp686C18211; KIAA0075; MGC104598; RP1-177P22.2
E2F-3; E2F3; KIAA0075