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|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length human protein.|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26624 detects E2F4 from human and rat samples.
MA1-26624 has been successfully used in immunohistochemistry (paraffin) procedures.
The MA1-26624 immunogen is recombinant, full length human protein.
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases.
MA1-26624 was used in immunohistochemistry - paraffin section to study the relationship between the immunohistochemical expression of E2F1, E2F2 and E2F4 and colorectal adenocarcinoma growth
|Xanthoulis A,Kotsinas A,Tiniakos D,Fiska A,Tentes AA,Kyroudi A,Kittas C,Gorgoulis V||Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry (22:471)||2014|
Effects of isotretinoin on spermatogenesis of rats.
MA1-26624 was used in immunohistochemistry to investigate the influence of isotretinoin administration on rodent spermatogenic activity
|Gencoglan G,Tosun M||Cutaneous and ocular toxicology (30:55)||2011|
High-resolution analysis of 16q22.1 in breast carcinoma using DNA amplifiable probes (multiplex amplifiable probe hybridization technique) and immunohistochemistry.
MA1-26624 was used in immunohistochemistry to study 16q22.1 in breast carcinoma and identify potential tumor suppressor genes
|Rakha EA,Armour JA,Pinder SE,Paish CE,Ellis IO||International journal of cancer (114:720)||2005|
Classical Hodgkin lymphoma is characterized by high constitutive expression of activating transcription factor 3 (ATF3), which promotes viability of Hodgkin/Reed-Sternberg cells.
MA1-26624 was used in western blot to study the critical role of activating transcription factor 3 in the pathogenesis classical Hodgkin's lymphoma
|Janz M,Hummel M,Truss M,Wollert-Wulf B,Mathas S,Jöhrens K,Hagemeier C,Bommert K,Stein H,Dörken B,Bargou RC||Blood (107:2536)||2006|
The adenovirus E4-6/7 protein directs nuclear localization of E2F-4 via an arginine-rich motif.
MA1-26624 was used in western blot to study the role of adenovirus E4-6/7 protein in directing nuclear localization of E2F-4
|Schaley JE,Polonskaia M,Hearing P||Journal of virology (79:2301)||2005|
p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2.
MA1-26624 was used in western blot to study the role of Skp2 in the mechanism by which p107 inhibits G1/S phase progression
|Rodier G,Makris C,Coulombe P,Scime A,Nakayama K,Nakayama KI,Meloche S||The Journal of cell biology (168:55)||2005|
E2F transcription factor 4, p107/p130-binding; p107/p130-binding protein; transcription factor E2F4