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Western blot detection of Transferrin in (lane 1) human Transferrin, (lane 2) control with no antibody using a Transferrin polyclonal antibody (Product # PA1-21594) at a dilution of 1:1000 overnight at 4°C. Secondary detection was performed using a Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at room temperature and blocked for 30 min at room temperature. Predicted/Observed size: 76-81 kDa. 76 kDa for Transferrin. Other band(s): Transferrin slice varients and isoforms.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Transferrin [human serum]|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21594 detects Transferrin in Human samples.
PA1-21594 has been successfully used in ELISA, Immunoprecipitation and Western Blot procedures.
PA1-21594 immunogen corresponds to Transferrin [human serum].
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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