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Invitrogen
Transferrin Receptor Monoclonal Antibody (236-15375)
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FIGURE: 1 / 9
Transferrin Receptor Antibody (A-11130) in ICC/IF
Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor (236-15375) Mouse Monoclonal Antibody (Product # A-11130) at 2 µg/mL in 0.1% BSA... View More
Product Details
A-11130
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
236-15375
Immunogen
human Transferrin Receptor
Conjugate
Form
Lyophilized
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
The anti-human transferrin receptor antibody should be useful for the localization of transferrin receptors by immunofluorescence techniques. This antibody recognizes the human transferrin receptor and does not block binding of transferrin to the receptor.
Although fluorescent transferrin conjugates have been widely used to study transferrin receptor-mediated endocytosis, antibodies to the transferrin receptor have proven to be useful for localizing transferrin receptors, studying endocytic recycling pathways and performing enzyme-linked immunosorbent assays (ELISAs).
Upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2 mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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