|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||See 3 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse , Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human Transferrin Receptor|
Storage and reconstitution: upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD71; p90; T9; TFR; TFR1; TR; Transferrin receptor protein 1; TRFR
Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR
UniProt ID: (Human) P02786
Entrez Gene ID: (Human) 7037
Molecular Function: receptor
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