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Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor (236-15375) Mouse Monoclonal Antibody (A11130) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human Transferrin Receptor|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Storage and reconstitution: upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity.
A-11130 was used in western blot to investigate the role of Fbxo2 in regulating NMDAR subunits in the brain.
|Atkin G,Moore S,Lu Y,Nelson RF,Tipper N,Rajpal G,Hunt J,Tennant W,Hell JW,Murphy GG,Paulson H||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:6165)||2015|
CD71; p90; T9; TFR; TFR1; TR; Transferrin receptor protein 1; TRFR
CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR