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Visualization of transferrin and transferrin receptors in A431 cells. A431 cells incubated with green-fluorescent Alexa Fluor® 488 transferrin (Cat. no. T13342), then fixed and permeabilized. Transferrin receptors were identified with anti–transferrin receptor, mouse IgG1 monoclonal antibody (Cat. no. A11130) and visualized with red-fluorescent Alexa Fluor® 555 goat anti–mouse IgG antibody (Cat. no. A21422). Yellow fluorescence indicates regions of co-localization. Nuclei were stained with DAPI (Cat. no. D1306, D3571, D21490).
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human Transferrin Receptor|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1.0 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Storage and reconstitution: upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity.
A-11130 was used in western blot to investigate the role of Fbxo2 in regulating NMDAR subunits in the brain.
|Atkin G,Moore S,Lu Y,Nelson RF,Tipper N,Rajpal G,Hunt J,Tennant W,Hell JW,Murphy GG,Paulson H||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:6165)||2015|
CD71; TFR; TFR1; transferrin receptor protein 1; TRFR
CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR